20170618_1831_phos1_S8.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.59.09
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fb32d50a020ef95ff2f4ae260317629a",
"name": "M1_20170618_1831_dsRNA_S8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:59:31"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8502 (max 8503) in '8502'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9998
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.00844e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 8 mean_circ: 0.947585
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Ran for 385050 frames Cell Events: 6519 mean_circ: 0.940737
Ran for 385550 frames Cell Events: 6525 mean_circ: 0.94075
Ran for 386050 frames Cell Events: 6531 mean_circ: 0.94076
Ran for 386550 frames Cell Events: 6537 mean_circ: 0.940754
Ran for 387050 frames Cell Events: 6547 mean_circ: 0.940748
Ran for 388050 frames Cell Events: 6565 mean_circ: 0.9408
Ran for 388550 frames Cell Events: 6574 mean_circ: 0.940816
Ran for 389050 frames Cell Events: 6580 mean_circ: 0.940809
Ran for 389550 frames Cell Events: 6585 mean_circ: 0.940823
Ran for 390050 frames Cell Events: 6590 mean_circ: 0.940838
Ran for 390550 frames Cell Events: 6593 mean_circ: 0.940847
Ran for 391050 frames Cell Events: 6603 mean_circ: 0.940868
Ran for 391550 frames Cell Events: 6611 mean_circ: 0.940888
Ran for 392050 frames Cell Events: 6615 mean_circ: 0.940897
Ran for 392550 frames Cell Events: 6622 mean_circ: 0.940894
Ran for 393050 frames Cell Events: 6629 mean_circ: 0.940872
Ran for 393550 frames Cell Events: 6642 mean_circ: 0.940902
Ran for 394050 frames Cell Events: 6646 mean_circ: 0.940902
Ran for 394550 frames Cell Events: 6653 mean_circ: 0.940897
Ran for 395050 frames Cell Events: 6656 mean_circ: 0.940902
Ran for 395550 frames Cell Events: 6664 mean_circ: 0.940896
Ran for 396050 frames Cell Events: 6670 mean_circ: 0.940905
Ran for 398550 frames Cell Events: 6706 mean_circ: 0.940944
Ran for 399050 frames Cell Events: 6709 mean_circ: 0.94095
Ran for 400050 frames Cell Events: 6726 mean_circ: 0.940941
Ran for 401050 frames Cell Events: 6740 mean_circ: 0.940944
Ran for 401550 frames Cell Events: 6748 mean_circ: 0.940953
Ran for 402550 frames Cell Events: 6768 mean_circ: 0.940962
Ran for 403050 frames Cell Events: 6774 mean_circ: 0.940958
Ran for 403550 frames Cell Events: 6782 mean_circ: 0.94095
Ran for 404050 frames Cell Events: 6793 mean_circ: 0.940969
Ran for 404550 frames Cell Events: 6796 mean_circ: 0.940974
Ran for 405050 frames Cell Events: 6805 mean_circ: 0.940986
Ran for 405550 frames Cell Events: 6817 mean_circ: 0.940968
Ran for 406050 frames Cell Events: 6823 mean_circ: 0.940969
Ran for 406550 frames Cell Events: 6831 mean_circ: 0.940987
Ran for 407050 frames Cell Events: 6836 mean_circ: 0.940996
Ran for 407550 frames Cell Events: 6844 mean_circ: 0.941028
Ran for 408050 frames Cell Events: 6851 mean_circ: 0.941022
Ran for 408550 frames Cell Events: 6858 mean_circ: 0.94102
Ran for 409050 frames Cell Events: 6868 mean_circ: 0.941014
Ran for 409550 frames Cell Events: 6876 mean_circ: 0.941044
Ran for 410050 frames Cell Events: 6885 mean_circ: 0.941062
Ran for 410550 frames Cell Events: 6897 mean_circ: 0.941045
Ran for 411050 frames Cell Events: 6905 mean_circ: 0.941036
Ran for 411550 frames Cell Events: 6914 mean_circ: 0.941038
Ran for 412050 frames Cell Events: 6920 mean_circ: 0.940996
Ran for 412550 frames Cell Events: 6927 mean_circ: 0.940996
Ran for 413050 frames Cell Events: 6937 mean_circ: 0.940988
Ran for 413550 frames Cell Events: 6947 mean_circ: 0.940987
Ran for 414550 frames Cell Events: 6965 mean_circ: 0.940989
Ran for 415550 frames Cell Events: 6995 mean_circ: 0.940974
Ran for 416550 frames Cell Events: 7020 mean_circ: 0.940953
Ran for 417550 frames Cell Events: 7043 mean_circ: 0.940987
Ran for 418550 frames Cell Events: 7062 mean_circ: 0.940991
Ran for 419550 frames Cell Events: 7078 mean_circ: 0.940976
Ran for 420550 frames Cell Events: 7104 mean_circ: 0.940967
Ran for 421050 frames Cell Events: 7116 mean_circ: 0.940956
Ran for 421550 frames Cell Events: 7121 mean_circ: 0.940971
Ran for 422050 frames Cell Events: 7131 mean_circ: 0.940946
Ran for 422550 frames Cell Events: 7141 mean_circ: 0.94094
Ran for 423050 frames Cell Events: 7146 mean_circ: 0.940952
Ran for 423550 frames Cell Events: 7163 mean_circ: 0.940964
Ran for 424050 frames Cell Events: 7171 mean_circ: 0.940941
Ran for 424550 frames Cell Events: 7178 mean_circ: 0.94093
Ran for 426050 frames Cell Events: 7204 mean_circ: 0.940912
Ran for 426550 frames Cell Events: 7209 mean_circ: 0.940934
Ran for 427050 frames Cell Events: 7215 mean_circ: 0.940938
Ran for 427550 frames Cell Events: 7220 mean_circ: 0.940931
Ran for 428550 frames Cell Events: 7248 mean_circ: 0.940961
Ran for 429050 frames Cell Events: 7262 mean_circ: 0.94095
Ran for 429550 frames Cell Events: 7265 mean_circ: 0.940946
Ran for 430050 frames Cell Events: 7274 mean_circ: 0.940947
Ran for 431050 frames Cell Events: 7293 mean_circ: 0.940957
Ran for 431550 frames Cell Events: 7300 mean_circ: 0.940949
Ran for 432050 frames Cell Events: 7306 mean_circ: 0.940934
Ran for 432550 frames Cell Events: 7316 mean_circ: 0.940934
Ran for 433050 frames Cell Events: 7330 mean_circ: 0.940924
Ran for 433550 frames Cell Events: 7338 mean_circ: 0.94092
Ran for 434550 frames Cell Events: 7357 mean_circ: 0.940932
Ran for 435050 frames Cell Events: 7365 mean_circ: 0.94091
Ran for 435550 frames Cell Events: 7369 mean_circ: 0.940901
Ran for 436550 frames Cell Events: 7392 mean_circ: 0.940896
Ran for 437050 frames Cell Events: 7404 mean_circ: 0.94092
Ran for 438050 frames Cell Events: 7427 mean_circ: 0.940921
Ran for 439050 frames Cell Events: 7452 mean_circ: 0.940921
Ran for 439550 frames Cell Events: 7459 mean_circ: 0.940916
Ran for 440050 frames Cell Events: 7460 mean_circ: 0.940921
Ran for 440550 frames Cell Events: 7465 mean_circ: 0.940934
Ran for 441550 frames Cell Events: 7489 mean_circ: 0.940879
Ran for 443050 frames Cell Events: 7510 mean_circ: 0.940897
Ran for 444050 frames Cell Events: 7527 mean_circ: 0.94088
Ran for 445550 frames Cell Events: 7549 mean_circ: 0.940909
Ran for 447050 frames Cell Events: 7586 mean_circ: 0.940897
Ran for 448050 frames Cell Events: 7602 mean_circ: 0.940909
Ran for 448550 frames Cell Events: 7604 mean_circ: 0.940904
Ran for 449050 frames Cell Events: 7612 mean_circ: 0.940912
Ran for 449550 frames Cell Events: 7616 mean_circ: 0.940914
Ran for 450050 frames Cell Events: 7626 mean_circ: 0.940926
Ran for 451050 frames Cell Events: 7640 mean_circ: 0.940949
Ran for 451550 frames Cell Events: 7646 mean_circ: 0.940964
Ran for 453550 frames Cell Events: 7680 mean_circ: 0.940967
Ran for 454050 frames Cell Events: 7683 mean_circ: 0.940962
Ran for 454550 frames Cell Events: 7690 mean_circ: 0.940974
Ran for 455050 frames Cell Events: 7695 mean_circ: 0.940955
Ran for 455550 frames Cell Events: 7704 mean_circ: 0.940924
Ran for 456050 frames Cell Events: 7708 mean_circ: 0.940939
Ran for 456550 frames Cell Events: 7718 mean_circ: 0.940965
Ran for 457050 frames Cell Events: 7722 mean_circ: 0.940974
Ran for 457550 frames Cell Events: 7733 mean_circ: 0.940977
Ran for 458050 frames Cell Events: 7746 mean_circ: 0.941004
Ran for 458550 frames Cell Events: 7752 mean_circ: 0.941019
Ran for 459550 frames Cell Events: 7768 mean_circ: 0.94103
Ran for 460050 frames Cell Events: 7774 mean_circ: 0.941041
Ran for 460550 frames Cell Events: 7778 mean_circ: 0.941031
Ran for 461050 frames Cell Events: 7785 mean_circ: 0.941036
Ran for 461550 frames Cell Events: 7793 mean_circ: 0.940998
Ran for 462050 frames Cell Events: 7798 mean_circ: 0.940993
Ran for 463550 frames Cell Events: 7824 mean_circ: 0.940974
Ran for 464050 frames Cell Events: 7833 mean_circ: 0.940959
Ran for 465050 frames Cell Events: 7879 mean_circ: 0.94097
Ran for 465550 frames Cell Events: 7883 mean_circ: 0.940789
Ran for 466050 frames Cell Events: 7890 mean_circ: 0.940795
Ran for 466550 frames Cell Events: 7903 mean_circ: 0.940774
Ran for 467050 frames Cell Events: 7911 mean_circ: 0.940787
Ran for 467550 frames Cell Events: 7915 mean_circ: 0.94079
Ran for 468050 frames Cell Events: 7920 mean_circ: 0.9408
Ran for 469050 frames Cell Events: 7936 mean_circ: 0.940789
Ran for 469550 frames Cell Events: 7947 mean_circ: 0.940788
Ran for 470050 frames Cell Events: 7948 mean_circ: 0.940785
Ran for 471050 frames Cell Events: 7962 mean_circ: 0.940788
Ran for 472050 frames Cell Events: 7977 mean_circ: 0.940791
Ran for 472550 frames Cell Events: 7983 mean_circ: 0.940801
Ran for 473050 frames Cell Events: 7997 mean_circ: 0.940793
Ran for 473550 frames Cell Events: 8004 mean_circ: 0.940796
Ran for 474050 frames Cell Events: 8006 mean_circ: 0.940783
Ran for 475050 frames Cell Events: 8021 mean_circ: 0.940759
Ran for 475550 frames Cell Events: 8031 mean_circ: 0.940771
Ran for 476050 frames Cell Events: 8042 mean_circ: 0.940782
Ran for 476550 frames Cell Events: 8051 mean_circ: 0.940775
Ran for 477050 frames Cell Events: 8065 mean_circ: 0.940763
Ran for 477550 frames Cell Events: 8072 mean_circ: 0.940766
Ran for 478050 frames Cell Events: 8081 mean_circ: 0.940787
Ran for 479050 frames Cell Events: 8106 mean_circ: 0.940792
Ran for 479550 frames Cell Events: 8117 mean_circ: 0.940794
Ran for 480550 frames Cell Events: 8131 mean_circ: 0.940813
Ran for 481050 frames Cell Events: 8137 mean_circ: 0.940811
Ran for 482050 frames Cell Events: 8157 mean_circ: 0.940843
Ran for 482550 frames Cell Events: 8159 mean_circ: 0.940845
Ran for 484550 frames Cell Events: 8213 mean_circ: 0.940835
Ran for 485550 frames Cell Events: 8236 mean_circ: 0.94081
Ran for 486050 frames Cell Events: 8245 mean_circ: 0.94082
Ran for 486550 frames Cell Events: 8253 mean_circ: 0.940808
Ran for 487050 frames Cell Events: 8268 mean_circ: 0.940791
Ran for 487550 frames Cell Events: 8273 mean_circ: 0.940802
Ran for 488050 frames Cell Events: 8285 mean_circ: 0.940766
Ran for 488550 frames Cell Events: 8295 mean_circ: 0.940779
Ran for 489050 frames Cell Events: 8303 mean_circ: 0.940788
Ran for 489550 frames Cell Events: 8308 mean_circ: 0.940777
Ran for 490050 frames Cell Events: 8314 mean_circ: 0.940774
Ran for 490550 frames Cell Events: 8317 mean_circ: 0.940759
Ran for 491050 frames Cell Events: 8321 mean_circ: 0.940751
Ran for 491550 frames Cell Events: 8323 mean_circ: 0.940756
Ran for 492050 frames Cell Events: 8333 mean_circ: 0.940777
Ran for 494050 frames Cell Events: 8373 mean_circ: 0.940782
Ran for 494550 frames Cell Events: 8379 mean_circ: 0.94078
Ran for 495050 frames Cell Events: 8386 mean_circ: 0.940769
Ran for 495550 frames Cell Events: 8393 mean_circ: 0.940778
Ran for 496050 frames Cell Events: 8397 mean_circ: 0.940778
Ran for 496550 frames Cell Events: 8405 mean_circ: 0.940776
Ran for 497050 frames Cell Events: 8413 mean_circ: 0.940783
Ran for 498050 frames Cell Events: 8429 mean_circ: 0.940796
Ran for 498550 frames Cell Events: 8440 mean_circ: 0.940819
Ran for 499050 frames Cell Events: 8448 mean_circ: 0.940814
Ran for 500550 frames Cell Events: 8477 mean_circ: 0.940831
Ran for 501050 frames Cell Events: 8485 mean_circ: 0.940828
Ran for 501550 frames Cell Events: 8493 mean_circ: 0.940834
End this measurment..
Stopping time: 4.01095e+008
****************************************
Measurement finished
Mean Circularitiy: 0.94085
Mean Area: 637.11
Mean FL-1 intensity: 3031.44
Mean FL-2 intensity: 3791.48
Mean FL-3 intensity: 0
frames processed: 501772
Total Time: 251052ms
Number of Runs (new bg img) 0
Frames collected: 501930
Skipped frames: 4972
Rescued Frames: 1031840
Samples DAQ: 125483621
Samples written: 250965172
Samples per Channel: 125482586
FPS: 1999.31
Samples per second: 499827
Expected samples/s: 500000
Events: 8503
Events per second: 33.8695
jumped back (PMT): 6330
#Frames read, frequency
# 0 frames :676005
# 1 frames :299128
# 2 frames :7179
# 3 frames :3897
# 4 frames :2435
# 5 frames :1482
# 6 frames :979
# 7 frames :1848
# 8 frames :1565
# 9 frames :1209
# 10 frames :1101
# 11 frames :1134
# 12 frames :1267
# 13 frames :840
# 14 frames :573
# 15 frames :427
# 16 frames :496
# 17 frames :566
# 18 frames :425
# 19 frames :280
# 20 frames :174
# 21 frames :169
# 22 frames :138
# 23 frames :116
# 24 frames :80
# 25 frames :50
# 26 frames :38
# 27 frames :29
# 28 frames :16
# 29 frames :16
# 30 frames :17
# 31 frames :14
# 32 frames :13
# 33 frames :18
# 34 frames :10
# 35 frames :3
# 36 frames :5
# 37 frames :5
# 38 frames :3
# 39 frames :5
# 40 frames :5
# 41 frames :2
# 42 frames :1
# 43 frames :4
# 44 frames :2
# 45 frames :1
# 46 frames :3
# 47 frames :1
# 49 frames :2
# 50 frames :2
# 51 frames :2
# 56 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9998
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 1 month ago |
etag | 859f861b06776b7d6ac1ce92ce92d207 |
format | RT-FDC |
id | c838a7d8-407b-4824-9cac-7c18371fe18a |
metadata modified | 1 month ago |
mimetype | RT-DC |
package id | b7981a8e-ca3a-4470-9748-45fa6ba75c5d |
position | 2 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5d2846fc-f12d-45de-96d1-e1eea06e16c7/resource/c83/8a7/d8-407b-4824-9cac-7c18371fe18a |
sha256 | 8ebf87d34a5997e9abe6d792ed803454ce3ac62664c01609fc3d55f0a6e7cb0d |
size | 148.4 MiB |
dc:experiment:date | 2017-06-18 |
dc:experiment:event count | 8,501 |
dc:experiment:run identifier | None-fcc1 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170618_1831_dsRNA_S8_col |
dc:experiment:time | 18:31:40 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |