Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.16.32
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ecdfd2c8e60dd36d32b18ce04b2b1f4e",
"name": "M1_20170608_1344_dsRNA_T3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:16:52"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8129 (max 8130) in '8129'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7198
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.0929e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.945681
Ran for 3050 frames Cell Events: 105 mean_circ: 0.937831
Ran for 3550 frames Cell Events: 120 mean_circ: 0.934935
Ran for 4050 frames Cell Events: 139 mean_circ: 0.935164
Ran for 4550 frames Cell Events: 152 mean_circ: 0.93627
Ran for 5050 frames Cell Events: 164 mean_circ: 0.936499
Ran for 5550 frames Cell Events: 177 mean_circ: 0.936233
Ran for 6050 frames Cell Events: 192 mean_circ: 0.935162
Ran for 6550 frames Cell Events: 206 mean_circ: 0.934555
Ran for 7050 frames Cell Events: 215 mean_circ: 0.935227
Ran for 7550 frames Cell Events: 224 mean_circ: 0.936381
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Ran for 11050 frames Cell Events: 321 mean_circ: 0.935048
Ran for 12050 frames Cell Events: 351 mean_circ: 0.934681
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Ran for 17050 frames Cell Events: 509 mean_circ: 0.9334
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Ran for 64550 frames Cell Events: 1932 mean_circ: 0.933189
Ran for 65050 frames Cell Events: 1941 mean_circ: 0.933209
Ran for 66050 frames Cell Events: 1971 mean_circ: 0.93323
Ran for 67050 frames Cell Events: 2009 mean_circ: 0.933204
Ran for 67550 frames Cell Events: 2023 mean_circ: 0.933086
Ran for 69050 frames Cell Events: 2079 mean_circ: 0.933136
Ran for 69550 frames Cell Events: 2093 mean_circ: 0.933216
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Ran for 73550 frames Cell Events: 2229 mean_circ: 0.933165
Ran for 75050 frames Cell Events: 2272 mean_circ: 0.933071
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Ran for 76050 frames Cell Events: 2316 mean_circ: 0.93313
Ran for 76550 frames Cell Events: 2330 mean_circ: 0.933156
Ran for 77050 frames Cell Events: 2342 mean_circ: 0.933186
Ran for 77550 frames Cell Events: 2353 mean_circ: 0.933092
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Ran for 82050 frames Cell Events: 2505 mean_circ: 0.933436
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Ran for 83050 frames Cell Events: 2531 mean_circ: 0.933382
Ran for 83550 frames Cell Events: 2543 mean_circ: 0.933412
Ran for 84050 frames Cell Events: 2556 mean_circ: 0.933538
Ran for 84550 frames Cell Events: 2579 mean_circ: 0.933566
Ran for 85050 frames Cell Events: 2591 mean_circ: 0.93354
Ran for 85550 frames Cell Events: 2620 mean_circ: 0.933559
Ran for 86550 frames Cell Events: 2651 mean_circ: 0.933494
Ran for 87550 frames Cell Events: 2715 mean_circ: 0.933552
Ran for 88050 frames Cell Events: 2740 mean_circ: 0.933609
Ran for 88550 frames Cell Events: 2759 mean_circ: 0.933611
Ran for 89050 frames Cell Events: 2771 mean_circ: 0.933518
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Ran for 90050 frames Cell Events: 2796 mean_circ: 0.933379
Ran for 90550 frames Cell Events: 2810 mean_circ: 0.933503
Ran for 91050 frames Cell Events: 2824 mean_circ: 0.933542
Ran for 92050 frames Cell Events: 2846 mean_circ: 0.933644
Ran for 93050 frames Cell Events: 2874 mean_circ: 0.933567
Ran for 93550 frames Cell Events: 2890 mean_circ: 0.933551
Ran for 94050 frames Cell Events: 2899 mean_circ: 0.933543
Ran for 94550 frames Cell Events: 2906 mean_circ: 0.933548
Ran for 95050 frames Cell Events: 2915 mean_circ: 0.933514
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Ran for 96050 frames Cell Events: 2955 mean_circ: 0.933608
Ran for 96550 frames Cell Events: 2968 mean_circ: 0.933597
Ran for 98550 frames Cell Events: 3031 mean_circ: 0.933566
Ran for 99050 frames Cell Events: 3052 mean_circ: 0.933537
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Ran for 100550 frames Cell Events: 3100 mean_circ: 0.933682
Ran for 101050 frames Cell Events: 3114 mean_circ: 0.93368
Ran for 102050 frames Cell Events: 3155 mean_circ: 0.933673
Ran for 102550 frames Cell Events: 3172 mean_circ: 0.933657
Ran for 103550 frames Cell Events: 3195 mean_circ: 0.933536
Ran for 104550 frames Cell Events: 3232 mean_circ: 0.933653
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Ran for 107050 frames Cell Events: 3315 mean_circ: 0.933623
Ran for 107550 frames Cell Events: 3330 mean_circ: 0.933553
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Ran for 116050 frames Cell Events: 3592 mean_circ: 0.933752
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Ran for 120050 frames Cell Events: 3723 mean_circ: 0.93409
Ran for 120550 frames Cell Events: 3742 mean_circ: 0.934145
Ran for 121050 frames Cell Events: 3754 mean_circ: 0.934189
Ran for 121550 frames Cell Events: 3770 mean_circ: 0.934178
Ran for 123050 frames Cell Events: 3825 mean_circ: 0.934186
Ran for 124050 frames Cell Events: 3855 mean_circ: 0.934271
Ran for 124550 frames Cell Events: 3871 mean_circ: 0.934307
Ran for 125050 frames Cell Events: 3890 mean_circ: 0.934286
Ran for 125550 frames Cell Events: 3906 mean_circ: 0.934165
Ran for 126050 frames Cell Events: 3931 mean_circ: 0.934164
Ran for 127550 frames Cell Events: 3984 mean_circ: 0.934182
Ran for 128050 frames Cell Events: 4003 mean_circ: 0.934105
Ran for 128550 frames Cell Events: 4026 mean_circ: 0.934085
Ran for 129050 frames Cell Events: 4035 mean_circ: 0.934057
Ran for 131050 frames Cell Events: 4092 mean_circ: 0.934188
Ran for 131550 frames Cell Events: 4112 mean_circ: 0.934088
Ran for 133050 frames Cell Events: 4161 mean_circ: 0.934092
Ran for 133550 frames Cell Events: 4179 mean_circ: 0.934167
Ran for 134050 frames Cell Events: 4192 mean_circ: 0.934195
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Ran for 136550 frames Cell Events: 4262 mean_circ: 0.93436
Ran for 138050 frames Cell Events: 4303 mean_circ: 0.934283
Ran for 139050 frames Cell Events: 4327 mean_circ: 0.934242
Ran for 139550 frames Cell Events: 4341 mean_circ: 0.934199
Ran for 140050 frames Cell Events: 4354 mean_circ: 0.934234
Ran for 141050 frames Cell Events: 4379 mean_circ: 0.934176
Ran for 141550 frames Cell Events: 4396 mean_circ: 0.934211
Ran for 142050 frames Cell Events: 4410 mean_circ: 0.934163
Ran for 143050 frames Cell Events: 4445 mean_circ: 0.934145
Ran for 144050 frames Cell Events: 4471 mean_circ: 0.934198
Ran for 144550 frames Cell Events: 4490 mean_circ: 0.934211
Ran for 145050 frames Cell Events: 4504 mean_circ: 0.93426
Ran for 145550 frames Cell Events: 4527 mean_circ: 0.93427
Ran for 147050 frames Cell Events: 4572 mean_circ: 0.934249
Ran for 147550 frames Cell Events: 4591 mean_circ: 0.934216
Ran for 149050 frames Cell Events: 4637 mean_circ: 0.934314
Ran for 150550 frames Cell Events: 4691 mean_circ: 0.934328
Ran for 152050 frames Cell Events: 4741 mean_circ: 0.934423
Ran for 152550 frames Cell Events: 4754 mean_circ: 0.934432
Ran for 153050 frames Cell Events: 4769 mean_circ: 0.934391
Ran for 154050 frames Cell Events: 4797 mean_circ: 0.934429
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Ran for 157050 frames Cell Events: 4897 mean_circ: 0.934503
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Ran for 183550 frames Cell Events: 5729 mean_circ: 0.934554
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Ran for 196050 frames Cell Events: 6116 mean_circ: 0.934505
Ran for 196550 frames Cell Events: 6135 mean_circ: 0.934494
Ran for 197050 frames Cell Events: 6140 mean_circ: 0.934495
Ran for 197550 frames Cell Events: 6149 mean_circ: 0.93453
Ran for 198550 frames Cell Events: 6181 mean_circ: 0.934507
Ran for 199550 frames Cell Events: 6213 mean_circ: 0.934494
Ran for 200050 frames Cell Events: 6230 mean_circ: 0.934447
Ran for 200550 frames Cell Events: 6241 mean_circ: 0.934446
Ran for 201550 frames Cell Events: 6272 mean_circ: 0.934453
Ran for 202050 frames Cell Events: 6294 mean_circ: 0.9344
Ran for 203050 frames Cell Events: 6336 mean_circ: 0.934349
Ran for 203550 frames Cell Events: 6356 mean_circ: 0.93433
Ran for 204050 frames Cell Events: 6371 mean_circ: 0.93428
Ran for 205050 frames Cell Events: 6410 mean_circ: 0.934286
Ran for 205550 frames Cell Events: 6433 mean_circ: 0.934313
Ran for 206050 frames Cell Events: 6450 mean_circ: 0.934306
Ran for 207550 frames Cell Events: 6500 mean_circ: 0.934318
Ran for 209050 frames Cell Events: 6549 mean_circ: 0.934277
Ran for 210050 frames Cell Events: 6578 mean_circ: 0.934265
Ran for 210550 frames Cell Events: 6595 mean_circ: 0.934308
Ran for 211550 frames Cell Events: 6637 mean_circ: 0.934263
Ran for 212050 frames Cell Events: 6654 mean_circ: 0.934255
Ran for 213050 frames Cell Events: 6687 mean_circ: 0.934252
Ran for 213550 frames Cell Events: 6701 mean_circ: 0.934265
Ran for 214050 frames Cell Events: 6720 mean_circ: 0.934289
Ran for 214550 frames Cell Events: 6731 mean_circ: 0.934275
Ran for 215050 frames Cell Events: 6745 mean_circ: 0.934287
Ran for 216050 frames Cell Events: 6774 mean_circ: 0.934264
Ran for 216550 frames Cell Events: 6789 mean_circ: 0.934249
Ran for 218050 frames Cell Events: 6832 mean_circ: 0.934202
Ran for 218550 frames Cell Events: 6845 mean_circ: 0.934214
Ran for 219550 frames Cell Events: 6877 mean_circ: 0.934202
Ran for 220050 frames Cell Events: 6901 mean_circ: 0.9342
Ran for 220550 frames Cell Events: 6919 mean_circ: 0.934182
Ran for 221050 frames Cell Events: 6932 mean_circ: 0.934152
Ran for 222550 frames Cell Events: 6987 mean_circ: 0.934205
Ran for 223050 frames Cell Events: 6996 mean_circ: 0.934193
Ran for 224550 frames Cell Events: 7040 mean_circ: 0.934158
Ran for 226050 frames Cell Events: 7091 mean_circ: 0.93416
Ran for 226550 frames Cell Events: 7109 mean_circ: 0.934163
Ran for 227050 frames Cell Events: 7123 mean_circ: 0.934141
Ran for 227550 frames Cell Events: 7137 mean_circ: 0.934138
Ran for 228550 frames Cell Events: 7179 mean_circ: 0.934114
Ran for 229050 frames Cell Events: 7194 mean_circ: 0.934093
Ran for 230050 frames Cell Events: 7224 mean_circ: 0.934088
Ran for 230550 frames Cell Events: 7239 mean_circ: 0.934059
Ran for 231050 frames Cell Events: 7254 mean_circ: 0.934048
Ran for 231550 frames Cell Events: 7272 mean_circ: 0.934065
Ran for 233050 frames Cell Events: 7337 mean_circ: 0.934128
Ran for 234550 frames Cell Events: 7398 mean_circ: 0.934146
Ran for 235050 frames Cell Events: 7416 mean_circ: 0.934123
Ran for 236050 frames Cell Events: 7448 mean_circ: 0.934095
Ran for 237050 frames Cell Events: 7510 mean_circ: 0.934063
Ran for 237550 frames Cell Events: 7529 mean_circ: 0.934056
Ran for 238550 frames Cell Events: 7568 mean_circ: 0.934045
Ran for 239050 frames Cell Events: 7587 mean_circ: 0.934023
Ran for 239550 frames Cell Events: 7604 mean_circ: 0.934009
Ran for 240050 frames Cell Events: 7618 mean_circ: 0.934043
Ran for 240550 frames Cell Events: 7636 mean_circ: 0.934083
Ran for 241550 frames Cell Events: 7674 mean_circ: 0.934068
Ran for 242050 frames Cell Events: 7684 mean_circ: 0.934046
Ran for 243050 frames Cell Events: 7711 mean_circ: 0.934072
Ran for 243550 frames Cell Events: 7732 mean_circ: 0.93407
Ran for 244050 frames Cell Events: 7754 mean_circ: 0.934096
Ran for 244550 frames Cell Events: 7768 mean_circ: 0.934107
Ran for 246050 frames Cell Events: 7818 mean_circ: 0.934072
Ran for 246550 frames Cell Events: 7829 mean_circ: 0.934103
Ran for 247050 frames Cell Events: 7840 mean_circ: 0.93409
Ran for 247550 frames Cell Events: 7854 mean_circ: 0.934111
Ran for 250550 frames Cell Events: 7975 mean_circ: 0.934192
Ran for 251550 frames Cell Events: 8005 mean_circ: 0.93419
Ran for 252050 frames Cell Events: 8019 mean_circ: 0.93418
Ran for 252550 frames Cell Events: 8027 mean_circ: 0.934201
Ran for 253550 frames Cell Events: 8056 mean_circ: 0.934215
Ran for 254550 frames Cell Events: 8082 mean_circ: 0.934218
Ran for 255050 frames Cell Events: 8105 mean_circ: 0.934201
Ran for 255550 frames Cell Events: 8120 mean_circ: 0.934203
End this measurment..
Stopping time: 2.09303e+009
****************************************
Measurement finished
Mean Circularitiy: 0.934181
Mean Area: 732.37
Mean FL-1 intensity: 7012.56
Mean FL-2 intensity: 9095.15
Mean FL-3 intensity: 0
frames processed: 255254
Total Time: 128014ms
Number of Runs (new bg img) 0
Frames collected: 255889
Skipped frames: 13018
Rescued Frames: 763114
Samples DAQ: 63972184
Samples written: 127944280
Samples per Channel: 63972140
FPS: 1998.91
Samples per second: 499726
Expected samples/s: 500000
Events: 8130
Events per second: 63.5085
jumped back (PMT): 4792
#Frames read, frequency
# 0 frames :355388
# 1 frames :137941
# 2 frames :5138
# 3 frames :2131
# 4 frames :1132
# 5 frames :509
# 6 frames :1222
# 7 frames :2038
# 8 frames :1230
# 9 frames :862
# 10 frames :1076
# 11 frames :1009
# 12 frames :654
# 13 frames :328
# 14 frames :163
# 15 frames :162
# 16 frames :127
# 17 frames :138
# 18 frames :88
# 19 frames :71
# 20 frames :68
# 21 frames :49
# 22 frames :43
# 23 frames :30
# 24 frames :25
# 25 frames :33
# 26 frames :13
# 27 frames :11
# 28 frames :17
# 29 frames :8
# 30 frames :10
# 31 frames :4
# 32 frames :2
# 33 frames :4
# 34 frames :2
# 35 frames :4
# 36 frames :4
# 37 frames :4
# 38 frames :1
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 49 frames :1
# 51 frames :1
# 52 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7198
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0