Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.41.04
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ad34f9f0c072ea672eef01540d90b8a5",
"name": "M1_20170620_1549_dsRNA_T8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:41:27"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9244 (max 9245) in '9244'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8873
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.63929e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 46 mean_circ: 0.954178
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Ran for 180550 frames Cell Events: 5799 mean_circ: 0.951577
Ran for 181050 frames Cell Events: 5813 mean_circ: 0.951559
Ran for 181550 frames Cell Events: 5830 mean_circ: 0.951462
Ran for 182050 frames Cell Events: 5844 mean_circ: 0.951498
Ran for 182550 frames Cell Events: 5857 mean_circ: 0.951529
Ran for 183050 frames Cell Events: 5877 mean_circ: 0.951544
Ran for 183550 frames Cell Events: 5892 mean_circ: 0.951543
Ran for 184050 frames Cell Events: 5909 mean_circ: 0.951569
Ran for 184550 frames Cell Events: 5932 mean_circ: 0.951576
Ran for 185050 frames Cell Events: 5947 mean_circ: 0.951588
Ran for 186050 frames Cell Events: 5987 mean_circ: 0.951575
Ran for 187050 frames Cell Events: 6015 mean_circ: 0.951586
Ran for 187550 frames Cell Events: 6030 mean_circ: 0.951575
Ran for 188050 frames Cell Events: 6049 mean_circ: 0.951552
Ran for 189550 frames Cell Events: 6090 mean_circ: 0.951545
Ran for 190550 frames Cell Events: 6122 mean_circ: 0.951562
Ran for 191050 frames Cell Events: 6135 mean_circ: 0.951568
Ran for 192050 frames Cell Events: 6176 mean_circ: 0.951542
Ran for 193050 frames Cell Events: 6202 mean_circ: 0.951536
Ran for 193550 frames Cell Events: 6214 mean_circ: 0.951491
Ran for 194050 frames Cell Events: 6223 mean_circ: 0.951504
Ran for 194550 frames Cell Events: 6244 mean_circ: 0.951503
Ran for 195050 frames Cell Events: 6266 mean_circ: 0.951524
Ran for 196050 frames Cell Events: 6298 mean_circ: 0.951519
Ran for 196550 frames Cell Events: 6316 mean_circ: 0.951544
Ran for 197050 frames Cell Events: 6327 mean_circ: 0.95151
Ran for 197550 frames Cell Events: 6347 mean_circ: 0.951529
Ran for 198050 frames Cell Events: 6363 mean_circ: 0.951534
Ran for 198550 frames Cell Events: 6383 mean_circ: 0.951472
Ran for 199550 frames Cell Events: 6421 mean_circ: 0.951505
Ran for 200050 frames Cell Events: 6432 mean_circ: 0.951509
Ran for 201050 frames Cell Events: 6468 mean_circ: 0.951536
Ran for 201550 frames Cell Events: 6479 mean_circ: 0.951538
Ran for 202550 frames Cell Events: 6515 mean_circ: 0.951528
Ran for 203050 frames Cell Events: 6534 mean_circ: 0.95151
Ran for 203550 frames Cell Events: 6555 mean_circ: 0.951521
Ran for 204050 frames Cell Events: 6573 mean_circ: 0.951531
Ran for 204550 frames Cell Events: 6592 mean_circ: 0.95155
Ran for 205550 frames Cell Events: 6625 mean_circ: 0.951558
Ran for 206050 frames Cell Events: 6638 mean_circ: 0.951582
Ran for 206550 frames Cell Events: 6658 mean_circ: 0.951591
Ran for 207050 frames Cell Events: 6671 mean_circ: 0.951611
Ran for 208050 frames Cell Events: 6705 mean_circ: 0.951595
Ran for 208550 frames Cell Events: 6719 mean_circ: 0.951568
Ran for 209550 frames Cell Events: 6752 mean_circ: 0.951572
Ran for 210050 frames Cell Events: 6765 mean_circ: 0.951559
Ran for 210550 frames Cell Events: 6776 mean_circ: 0.951569
Ran for 211050 frames Cell Events: 6793 mean_circ: 0.951585
Ran for 211550 frames Cell Events: 6803 mean_circ: 0.951593
Ran for 212050 frames Cell Events: 6814 mean_circ: 0.951551
Ran for 213050 frames Cell Events: 6847 mean_circ: 0.951504
Ran for 213550 frames Cell Events: 6865 mean_circ: 0.951499
Ran for 214050 frames Cell Events: 6882 mean_circ: 0.951505
Ran for 215050 frames Cell Events: 6914 mean_circ: 0.951492
Ran for 215550 frames Cell Events: 6929 mean_circ: 0.951463
Ran for 216050 frames Cell Events: 6941 mean_circ: 0.951468
Ran for 216550 frames Cell Events: 6952 mean_circ: 0.95146
Ran for 217550 frames Cell Events: 6983 mean_circ: 0.951508
Ran for 219050 frames Cell Events: 7033 mean_circ: 0.951532
Ran for 219550 frames Cell Events: 7044 mean_circ: 0.951542
Ran for 220050 frames Cell Events: 7057 mean_circ: 0.951501
Ran for 220550 frames Cell Events: 7074 mean_circ: 0.951514
Ran for 221050 frames Cell Events: 7089 mean_circ: 0.951523
Ran for 221550 frames Cell Events: 7120 mean_circ: 0.951522
Ran for 222550 frames Cell Events: 7157 mean_circ: 0.951507
Ran for 223550 frames Cell Events: 7194 mean_circ: 0.951462
Ran for 224050 frames Cell Events: 7211 mean_circ: 0.951478
Ran for 224550 frames Cell Events: 7232 mean_circ: 0.951479
Ran for 226050 frames Cell Events: 7274 mean_circ: 0.951482
Ran for 227050 frames Cell Events: 7313 mean_circ: 0.951474
Ran for 227550 frames Cell Events: 7337 mean_circ: 0.951483
Ran for 228050 frames Cell Events: 7351 mean_circ: 0.951491
Ran for 228550 frames Cell Events: 7368 mean_circ: 0.951516
Ran for 229050 frames Cell Events: 7387 mean_circ: 0.951536
Ran for 229550 frames Cell Events: 7398 mean_circ: 0.951537
Ran for 230050 frames Cell Events: 7411 mean_circ: 0.95153
Ran for 231550 frames Cell Events: 7469 mean_circ: 0.951566
Ran for 232050 frames Cell Events: 7483 mean_circ: 0.951574
Ran for 233050 frames Cell Events: 7518 mean_circ: 0.951597
Ran for 233550 frames Cell Events: 7540 mean_circ: 0.951561
Ran for 234050 frames Cell Events: 7560 mean_circ: 0.951552
Ran for 234550 frames Cell Events: 7582 mean_circ: 0.95152
Ran for 235550 frames Cell Events: 7613 mean_circ: 0.95147
Ran for 236050 frames Cell Events: 7635 mean_circ: 0.951502
Ran for 236550 frames Cell Events: 7654 mean_circ: 0.95152
Ran for 237050 frames Cell Events: 7672 mean_circ: 0.951526
Ran for 238550 frames Cell Events: 7733 mean_circ: 0.95154
Ran for 239550 frames Cell Events: 7768 mean_circ: 0.951541
Ran for 240050 frames Cell Events: 7791 mean_circ: 0.951568
Ran for 240550 frames Cell Events: 7809 mean_circ: 0.951578
Ran for 241050 frames Cell Events: 7825 mean_circ: 0.951599
Ran for 241550 frames Cell Events: 7837 mean_circ: 0.951604
Ran for 242050 frames Cell Events: 7864 mean_circ: 0.951573
Ran for 242550 frames Cell Events: 7878 mean_circ: 0.951538
Ran for 243050 frames Cell Events: 7885 mean_circ: 0.951536
Ran for 244050 frames Cell Events: 7914 mean_circ: 0.951542
Ran for 244550 frames Cell Events: 7925 mean_circ: 0.951523
Ran for 245550 frames Cell Events: 7963 mean_circ: 0.951508
Ran for 246050 frames Cell Events: 7984 mean_circ: 0.951498
Ran for 246550 frames Cell Events: 8001 mean_circ: 0.951509
Ran for 247050 frames Cell Events: 8014 mean_circ: 0.95151
Ran for 247550 frames Cell Events: 8030 mean_circ: 0.951498
Ran for 248050 frames Cell Events: 8040 mean_circ: 0.951497
Ran for 248550 frames Cell Events: 8055 mean_circ: 0.951493
Ran for 249050 frames Cell Events: 8070 mean_circ: 0.951517
Ran for 249550 frames Cell Events: 8081 mean_circ: 0.951528
Ran for 250050 frames Cell Events: 8101 mean_circ: 0.951543
Ran for 251050 frames Cell Events: 8129 mean_circ: 0.951555
Ran for 251550 frames Cell Events: 8136 mean_circ: 0.951569
Ran for 252550 frames Cell Events: 8176 mean_circ: 0.951605
Ran for 253550 frames Cell Events: 8224 mean_circ: 0.951605
Ran for 254050 frames Cell Events: 8239 mean_circ: 0.951629
Ran for 255050 frames Cell Events: 8279 mean_circ: 0.951635
Ran for 255550 frames Cell Events: 8292 mean_circ: 0.951658
Ran for 256050 frames Cell Events: 8304 mean_circ: 0.951662
Ran for 256550 frames Cell Events: 8320 mean_circ: 0.951647
Ran for 257050 frames Cell Events: 8333 mean_circ: 0.951665
Ran for 257550 frames Cell Events: 8350 mean_circ: 0.951676
Ran for 258550 frames Cell Events: 8384 mean_circ: 0.95164
Ran for 259550 frames Cell Events: 8425 mean_circ: 0.951656
Ran for 260050 frames Cell Events: 8442 mean_circ: 0.951639
Ran for 261050 frames Cell Events: 8476 mean_circ: 0.951624
Ran for 262050 frames Cell Events: 8509 mean_circ: 0.951606
Ran for 262550 frames Cell Events: 8528 mean_circ: 0.951609
Ran for 264050 frames Cell Events: 8596 mean_circ: 0.951617
Ran for 266050 frames Cell Events: 8668 mean_circ: 0.951581
Ran for 266550 frames Cell Events: 8681 mean_circ: 0.95159
Ran for 267050 frames Cell Events: 8698 mean_circ: 0.951613
Ran for 267550 frames Cell Events: 8707 mean_circ: 0.95162
Ran for 270050 frames Cell Events: 8779 mean_circ: 0.951539
Ran for 271050 frames Cell Events: 8812 mean_circ: 0.951561
Ran for 271550 frames Cell Events: 8828 mean_circ: 0.951551
Ran for 273050 frames Cell Events: 8875 mean_circ: 0.951539
Ran for 273550 frames Cell Events: 8890 mean_circ: 0.951523
Ran for 274050 frames Cell Events: 8900 mean_circ: 0.951497
Ran for 274550 frames Cell Events: 8916 mean_circ: 0.951496
Ran for 275050 frames Cell Events: 8929 mean_circ: 0.951462
Ran for 275550 frames Cell Events: 8937 mean_circ: 0.951466
Ran for 277050 frames Cell Events: 8979 mean_circ: 0.95147
Ran for 277550 frames Cell Events: 8997 mean_circ: 0.951471
Ran for 278050 frames Cell Events: 9012 mean_circ: 0.95149
Ran for 278550 frames Cell Events: 9024 mean_circ: 0.951485
Ran for 279550 frames Cell Events: 9062 mean_circ: 0.951482
Ran for 280050 frames Cell Events: 9074 mean_circ: 0.951497
Ran for 280550 frames Cell Events: 9092 mean_circ: 0.951499
Ran for 281050 frames Cell Events: 9114 mean_circ: 0.951486
Ran for 281550 frames Cell Events: 9133 mean_circ: 0.951438
Ran for 282050 frames Cell Events: 9147 mean_circ: 0.951414
Ran for 283050 frames Cell Events: 9184 mean_circ: 0.95141
Ran for 283550 frames Cell Events: 9207 mean_circ: 0.951416
Ran for 284050 frames Cell Events: 9225 mean_circ: 0.951416
Ran for 284550 frames Cell Events: 9234 mean_circ: 0.951433
End this measurment..
Stopping time: 5.64071e+008
****************************************
Measurement finished
Mean Circularitiy: 0.951423
Mean Area: 628.387
Mean FL-1 intensity: 3607.59
Mean FL-2 intensity: 4369.96
Mean FL-3 intensity: 0
frames processed: 284009
Total Time: 142566ms
Number of Runs (new bg img) 0
Frames collected: 284980
Skipped frames: 40249
Rescued Frames: 2220100
Samples DAQ: 71244978
Samples written: 142489780
Samples per Channel: 71244890
FPS: 1998.93
Samples per second: 499732
Expected samples/s: 500000
Events: 9245
Events per second: 64.847
jumped back (PMT): 7488
#Frames read, frequency
# 0 frames :385416
# 1 frames :162861
# 2 frames :7883
# 3 frames :1575
# 4 frames :1159
# 5 frames :344
# 6 frames :1579
# 7 frames :3613
# 8 frames :1926
# 9 frames :969
# 10 frames :592
# 11 frames :350
# 12 frames :408
# 13 frames :293
# 14 frames :212
# 15 frames :180
# 16 frames :163
# 17 frames :78
# 18 frames :70
# 19 frames :42
# 20 frames :34
# 21 frames :43
# 22 frames :25
# 23 frames :17
# 24 frames :18
# 25 frames :15
# 26 frames :9
# 27 frames :10
# 28 frames :8
# 29 frames :7
# 30 frames :4
# 31 frames :2
# 32 frames :5
# 33 frames :2
# 34 frames :3
# 35 frames :3
# 36 frames :3
# 37 frames :1
# 39 frames :3
# 43 frames :1
# 44 frames :1
# 46 frames :1
# 55 frames :1
# 62 frames :1
# 65 frames :1
# 73 frames :1
# 76 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8873
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0