Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.22.31
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fbd4a043e5447b392b16b9b011fe8544",
"name": "M1_20170608_1458_dsRNA_U1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:22:54"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8219 (max 8220) in '8219'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5575
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09735e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 49 mean_circ: 0.957379
Ran for 2050 frames Cell Events: 62 mean_circ: 0.959193
Ran for 3050 frames Cell Events: 99 mean_circ: 0.960355
Ran for 5050 frames Cell Events: 161 mean_circ: 0.961567
Ran for 6550 frames Cell Events: 201 mean_circ: 0.960302
Ran for 7050 frames Cell Events: 214 mean_circ: 0.958276
Ran for 8550 frames Cell Events: 263 mean_circ: 0.95646
Ran for 9050 frames Cell Events: 285 mean_circ: 0.955827
Ran for 9550 frames Cell Events: 304 mean_circ: 0.955148
Ran for 10050 frames Cell Events: 324 mean_circ: 0.955086
Ran for 10550 frames Cell Events: 342 mean_circ: 0.95481
Ran for 11050 frames Cell Events: 362 mean_circ: 0.955189
Ran for 12050 frames Cell Events: 389 mean_circ: 0.955266
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Ran for 13050 frames Cell Events: 425 mean_circ: 0.955086
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Ran for 14550 frames Cell Events: 464 mean_circ: 0.954832
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Ran for 73050 frames Cell Events: 2417 mean_circ: 0.953921
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Ran for 76550 frames Cell Events: 2532 mean_circ: 0.953997
Ran for 77050 frames Cell Events: 2545 mean_circ: 0.954051
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Ran for 79550 frames Cell Events: 2649 mean_circ: 0.95423
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Ran for 81050 frames Cell Events: 2687 mean_circ: 0.954305
Ran for 81550 frames Cell Events: 2705 mean_circ: 0.954288
Ran for 82050 frames Cell Events: 2717 mean_circ: 0.95434
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Ran for 84050 frames Cell Events: 2791 mean_circ: 0.95417
Ran for 84550 frames Cell Events: 2809 mean_circ: 0.95425
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Ran for 85550 frames Cell Events: 2841 mean_circ: 0.954262
Ran for 87050 frames Cell Events: 2892 mean_circ: 0.95434
Ran for 87550 frames Cell Events: 2901 mean_circ: 0.954334
Ran for 90050 frames Cell Events: 2984 mean_circ: 0.954312
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Ran for 91050 frames Cell Events: 3009 mean_circ: 0.954333
Ran for 92050 frames Cell Events: 3040 mean_circ: 0.954339
Ran for 92550 frames Cell Events: 3058 mean_circ: 0.954347
Ran for 93550 frames Cell Events: 3092 mean_circ: 0.954189
Ran for 94050 frames Cell Events: 3111 mean_circ: 0.954257
Ran for 94550 frames Cell Events: 3129 mean_circ: 0.95428
Ran for 95050 frames Cell Events: 3148 mean_circ: 0.95435
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Ran for 96050 frames Cell Events: 3175 mean_circ: 0.954413
Ran for 96550 frames Cell Events: 3184 mean_circ: 0.954426
Ran for 97550 frames Cell Events: 3202 mean_circ: 0.954456
Ran for 98550 frames Cell Events: 3242 mean_circ: 0.954404
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Ran for 100550 frames Cell Events: 3319 mean_circ: 0.954319
Ran for 101550 frames Cell Events: 3354 mean_circ: 0.954309
Ran for 102550 frames Cell Events: 3386 mean_circ: 0.954301
Ran for 103550 frames Cell Events: 3430 mean_circ: 0.954167
Ran for 104550 frames Cell Events: 3461 mean_circ: 0.954191
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Ran for 108550 frames Cell Events: 3589 mean_circ: 0.954178
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Ran for 120050 frames Cell Events: 4004 mean_circ: 0.954018
Ran for 120550 frames Cell Events: 4024 mean_circ: 0.954045
Ran for 121050 frames Cell Events: 4047 mean_circ: 0.954054
Ran for 121550 frames Cell Events: 4067 mean_circ: 0.953991
Ran for 123050 frames Cell Events: 4120 mean_circ: 0.95398
Ran for 123550 frames Cell Events: 4135 mean_circ: 0.954006
Ran for 124050 frames Cell Events: 4151 mean_circ: 0.953985
Ran for 125550 frames Cell Events: 4190 mean_circ: 0.954015
Ran for 126050 frames Cell Events: 4202 mean_circ: 0.954034
Ran for 126550 frames Cell Events: 4213 mean_circ: 0.95402
Ran for 127550 frames Cell Events: 4242 mean_circ: 0.953981
Ran for 128050 frames Cell Events: 4254 mean_circ: 0.954014
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Ran for 130050 frames Cell Events: 4308 mean_circ: 0.953898
Ran for 131050 frames Cell Events: 4349 mean_circ: 0.953865
Ran for 131550 frames Cell Events: 4366 mean_circ: 0.953861
Ran for 132050 frames Cell Events: 4375 mean_circ: 0.953851
Ran for 133550 frames Cell Events: 4435 mean_circ: 0.953761
Ran for 134050 frames Cell Events: 4443 mean_circ: 0.953668
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Ran for 141550 frames Cell Events: 4693 mean_circ: 0.953697
Ran for 142050 frames Cell Events: 4714 mean_circ: 0.953583
Ran for 142550 frames Cell Events: 4719 mean_circ: 0.953595
Ran for 143050 frames Cell Events: 4733 mean_circ: 0.953602
Ran for 143550 frames Cell Events: 4750 mean_circ: 0.953619
Ran for 144050 frames Cell Events: 4766 mean_circ: 0.953606
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Ran for 146050 frames Cell Events: 4826 mean_circ: 0.953578
Ran for 146550 frames Cell Events: 4839 mean_circ: 0.953556
Ran for 147050 frames Cell Events: 4859 mean_circ: 0.953575
Ran for 147550 frames Cell Events: 4874 mean_circ: 0.953611
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Ran for 172050 frames Cell Events: 5677 mean_circ: 0.953762
Ran for 172550 frames Cell Events: 5689 mean_circ: 0.953782
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Ran for 182550 frames Cell Events: 6013 mean_circ: 0.953823
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Ran for 196050 frames Cell Events: 6517 mean_circ: 0.953835
Ran for 197050 frames Cell Events: 6548 mean_circ: 0.953842
Ran for 198050 frames Cell Events: 6582 mean_circ: 0.953869
Ran for 198550 frames Cell Events: 6593 mean_circ: 0.953891
Ran for 199050 frames Cell Events: 6617 mean_circ: 0.953899
Ran for 199550 frames Cell Events: 6630 mean_circ: 0.953881
Ran for 200050 frames Cell Events: 6650 mean_circ: 0.953879
Ran for 201050 frames Cell Events: 6689 mean_circ: 0.953903
Ran for 201550 frames Cell Events: 6712 mean_circ: 0.953945
Ran for 202550 frames Cell Events: 6752 mean_circ: 0.953935
Ran for 203550 frames Cell Events: 6790 mean_circ: 0.95395
Ran for 204550 frames Cell Events: 6828 mean_circ: 0.953938
Ran for 205050 frames Cell Events: 6848 mean_circ: 0.953903
Ran for 205550 frames Cell Events: 6857 mean_circ: 0.953903
Ran for 206550 frames Cell Events: 6890 mean_circ: 0.95393
Ran for 207050 frames Cell Events: 6909 mean_circ: 0.953923
Ran for 207550 frames Cell Events: 6921 mean_circ: 0.953892
Ran for 208050 frames Cell Events: 6937 mean_circ: 0.953894
Ran for 208550 frames Cell Events: 6956 mean_circ: 0.953912
Ran for 209550 frames Cell Events: 6987 mean_circ: 0.953929
Ran for 210050 frames Cell Events: 7004 mean_circ: 0.953934
Ran for 210550 frames Cell Events: 7025 mean_circ: 0.95392
Ran for 211050 frames Cell Events: 7047 mean_circ: 0.953916
Ran for 211550 frames Cell Events: 7062 mean_circ: 0.953935
Ran for 212050 frames Cell Events: 7088 mean_circ: 0.953944
Ran for 212550 frames Cell Events: 7103 mean_circ: 0.953955
Ran for 213050 frames Cell Events: 7112 mean_circ: 0.953948
Ran for 214050 frames Cell Events: 7154 mean_circ: 0.953905
Ran for 214550 frames Cell Events: 7171 mean_circ: 0.953924
Ran for 215050 frames Cell Events: 7183 mean_circ: 0.953935
Ran for 216050 frames Cell Events: 7218 mean_circ: 0.953973
Ran for 216550 frames Cell Events: 7228 mean_circ: 0.953987
Ran for 217550 frames Cell Events: 7255 mean_circ: 0.953982
Ran for 219550 frames Cell Events: 7339 mean_circ: 0.953904
Ran for 220050 frames Cell Events: 7350 mean_circ: 0.953878
Ran for 220550 frames Cell Events: 7375 mean_circ: 0.95389
Ran for 221550 frames Cell Events: 7409 mean_circ: 0.95392
Ran for 222050 frames Cell Events: 7430 mean_circ: 0.953901
Ran for 222550 frames Cell Events: 7446 mean_circ: 0.953868
Ran for 223050 frames Cell Events: 7461 mean_circ: 0.953853
Ran for 223550 frames Cell Events: 7469 mean_circ: 0.953862
Ran for 224050 frames Cell Events: 7480 mean_circ: 0.953861
Ran for 224550 frames Cell Events: 7493 mean_circ: 0.953882
Ran for 225050 frames Cell Events: 7509 mean_circ: 0.953858
Ran for 226050 frames Cell Events: 7551 mean_circ: 0.953884
Ran for 226550 frames Cell Events: 7567 mean_circ: 0.953889
Ran for 227050 frames Cell Events: 7583 mean_circ: 0.953898
Ran for 228050 frames Cell Events: 7607 mean_circ: 0.953884
Ran for 228550 frames Cell Events: 7619 mean_circ: 0.953909
Ran for 229550 frames Cell Events: 7659 mean_circ: 0.953965
Ran for 230550 frames Cell Events: 7698 mean_circ: 0.95396
Ran for 231050 frames Cell Events: 7720 mean_circ: 0.953988
Ran for 231550 frames Cell Events: 7735 mean_circ: 0.953988
Ran for 232050 frames Cell Events: 7748 mean_circ: 0.953994
Ran for 234050 frames Cell Events: 7819 mean_circ: 0.95398
Ran for 235050 frames Cell Events: 7851 mean_circ: 0.953941
Ran for 235550 frames Cell Events: 7870 mean_circ: 0.953944
Ran for 236550 frames Cell Events: 7908 mean_circ: 0.953924
Ran for 238050 frames Cell Events: 7949 mean_circ: 0.95392
Ran for 238550 frames Cell Events: 7960 mean_circ: 0.953951
Ran for 239050 frames Cell Events: 7967 mean_circ: 0.953952
Ran for 240050 frames Cell Events: 8004 mean_circ: 0.95395
Ran for 240550 frames Cell Events: 8020 mean_circ: 0.95395
Ran for 241050 frames Cell Events: 8036 mean_circ: 0.95393
Ran for 241550 frames Cell Events: 8059 mean_circ: 0.953919
Ran for 242050 frames Cell Events: 8077 mean_circ: 0.95395
Ran for 242550 frames Cell Events: 8097 mean_circ: 0.95398
Ran for 243050 frames Cell Events: 8121 mean_circ: 0.953982
Ran for 243550 frames Cell Events: 8138 mean_circ: 0.953986
Ran for 244050 frames Cell Events: 8154 mean_circ: 0.953967
Ran for 245550 frames Cell Events: 8209 mean_circ: 0.953985
End this measurment..
Stopping time: 2.09747e+009
****************************************
Measurement finished
Mean Circularitiy: 0.953984
Mean Area: 589.008
Mean FL-1 intensity: 3831.29
Mean FL-2 intensity: 4304.16
Mean FL-3 intensity: 0
frames processed: 245787
Total Time: 122951ms
Number of Runs (new bg img) 0
Frames collected: 245787
Skipped frames: 27844
Rescued Frames: 1978588
Samples DAQ: 61446758
Samples written: 122893284
Samples per Channel: 61446642
FPS: 1999.06
Samples per second: 499765
Expected samples/s: 500000
Events: 8220
Events per second: 66.8559
jumped back (PMT): 4737
#Frames read, frequency
# 0 frames :341453
# 1 frames :132142
# 2 frames :5082
# 3 frames :1987
# 4 frames :1122
# 5 frames :402
# 6 frames :1600
# 7 frames :1690
# 8 frames :1227
# 9 frames :859
# 10 frames :1104
# 11 frames :918
# 12 frames :564
# 13 frames :294
# 14 frames :194
# 15 frames :162
# 16 frames :149
# 17 frames :134
# 18 frames :97
# 19 frames :71
# 20 frames :56
# 21 frames :45
# 22 frames :42
# 23 frames :30
# 24 frames :20
# 25 frames :24
# 26 frames :13
# 27 frames :20
# 28 frames :8
# 29 frames :11
# 30 frames :8
# 31 frames :7
# 32 frames :2
# 33 frames :2
# 34 frames :1
# 35 frames :1
# 36 frames :1
# 37 frames :1
# 38 frames :2
# 39 frames :1
# 40 frames :3
# 41 frames :1
# 43 frames :1
# 45 frames :2
# 46 frames :1
# 50 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5575
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0