Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_15.32.07
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f2b0b0add3ab1650a981b1b7be18ff55",
"name": "M1_20170609_1601_dsRNA_V6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:32:25"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7056 (max 7057) in '7056'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.8172
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.1875e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.924574
Ran for 2050 frames Cell Events: 67 mean_circ: 0.93139
Ran for 3050 frames Cell Events: 106 mean_circ: 0.930651
Ran for 4050 frames Cell Events: 134 mean_circ: 0.931575
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Ran for 5550 frames Cell Events: 173 mean_circ: 0.933844
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Ran for 6550 frames Cell Events: 209 mean_circ: 0.933681
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Ran for 10550 frames Cell Events: 324 mean_circ: 0.932049
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Ran for 13050 frames Cell Events: 381 mean_circ: 0.930331
Ran for 13550 frames Cell Events: 395 mean_circ: 0.93079
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Ran for 91550 frames Cell Events: 2767 mean_circ: 0.928593
Ran for 92550 frames Cell Events: 2798 mean_circ: 0.928662
Ran for 93050 frames Cell Events: 2818 mean_circ: 0.92871
Ran for 94550 frames Cell Events: 2850 mean_circ: 0.928784
Ran for 96050 frames Cell Events: 2886 mean_circ: 0.928758
Ran for 96550 frames Cell Events: 2892 mean_circ: 0.928779
Ran for 97550 frames Cell Events: 2927 mean_circ: 0.928842
Ran for 98050 frames Cell Events: 2935 mean_circ: 0.928825
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Ran for 99550 frames Cell Events: 2972 mean_circ: 0.928942
Ran for 100050 frames Cell Events: 2992 mean_circ: 0.928999
Ran for 100550 frames Cell Events: 3000 mean_circ: 0.928951
Ran for 101050 frames Cell Events: 3017 mean_circ: 0.928964
Ran for 102050 frames Cell Events: 3035 mean_circ: 0.928978
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Ran for 106550 frames Cell Events: 3168 mean_circ: 0.928972
Ran for 107050 frames Cell Events: 3183 mean_circ: 0.928952
Ran for 107550 frames Cell Events: 3204 mean_circ: 0.928873
Ran for 108550 frames Cell Events: 3238 mean_circ: 0.928852
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Ran for 112550 frames Cell Events: 3341 mean_circ: 0.928731
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Ran for 116050 frames Cell Events: 3439 mean_circ: 0.928751
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Ran for 122550 frames Cell Events: 3594 mean_circ: 0.928622
Ran for 124550 frames Cell Events: 3667 mean_circ: 0.928524
Ran for 125050 frames Cell Events: 3685 mean_circ: 0.928546
Ran for 126050 frames Cell Events: 3720 mean_circ: 0.928448
Ran for 126550 frames Cell Events: 3734 mean_circ: 0.928434
Ran for 127050 frames Cell Events: 3752 mean_circ: 0.928441
Ran for 127550 frames Cell Events: 3771 mean_circ: 0.928519
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Ran for 131050 frames Cell Events: 3859 mean_circ: 0.928592
Ran for 131550 frames Cell Events: 3872 mean_circ: 0.928577
Ran for 132050 frames Cell Events: 3876 mean_circ: 0.928551
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Ran for 136050 frames Cell Events: 3944 mean_circ: 0.928549
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Ran for 141550 frames Cell Events: 4076 mean_circ: 0.928792
Ran for 143050 frames Cell Events: 4101 mean_circ: 0.928835
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Ran for 147050 frames Cell Events: 4203 mean_circ: 0.928738
Ran for 147550 frames Cell Events: 4209 mean_circ: 0.928792
Ran for 148050 frames Cell Events: 4214 mean_circ: 0.928797
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Ran for 150550 frames Cell Events: 4274 mean_circ: 0.928947
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Ran for 151550 frames Cell Events: 4298 mean_circ: 0.928945
Ran for 152050 frames Cell Events: 4305 mean_circ: 0.928908
Ran for 152550 frames Cell Events: 4320 mean_circ: 0.928924
Ran for 153550 frames Cell Events: 4355 mean_circ: 0.928905
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Ran for 155550 frames Cell Events: 4425 mean_circ: 0.928892
Ran for 156550 frames Cell Events: 4459 mean_circ: 0.928897
Ran for 157050 frames Cell Events: 4473 mean_circ: 0.928894
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Ran for 162550 frames Cell Events: 4619 mean_circ: 0.929088
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Ran for 215050 frames Cell Events: 6205 mean_circ: 0.928603
Ran for 216550 frames Cell Events: 6270 mean_circ: 0.928574
Ran for 217050 frames Cell Events: 6284 mean_circ: 0.928531
Ran for 217550 frames Cell Events: 6290 mean_circ: 0.92856
Ran for 218550 frames Cell Events: 6323 mean_circ: 0.928539
Ran for 219550 frames Cell Events: 6358 mean_circ: 0.928527
Ran for 220550 frames Cell Events: 6380 mean_circ: 0.928544
Ran for 221050 frames Cell Events: 6398 mean_circ: 0.928553
Ran for 222050 frames Cell Events: 6422 mean_circ: 0.928603
Ran for 222550 frames Cell Events: 6434 mean_circ: 0.928592
Ran for 223050 frames Cell Events: 6447 mean_circ: 0.928576
Ran for 223550 frames Cell Events: 6458 mean_circ: 0.928606
Ran for 224050 frames Cell Events: 6469 mean_circ: 0.928629
Ran for 225050 frames Cell Events: 6497 mean_circ: 0.928681
Ran for 226050 frames Cell Events: 6521 mean_circ: 0.928706
Ran for 226550 frames Cell Events: 6534 mean_circ: 0.928675
Ran for 227050 frames Cell Events: 6545 mean_circ: 0.928653
Ran for 227550 frames Cell Events: 6560 mean_circ: 0.92866
Ran for 228050 frames Cell Events: 6575 mean_circ: 0.928643
Ran for 229050 frames Cell Events: 6609 mean_circ: 0.928663
Ran for 230050 frames Cell Events: 6640 mean_circ: 0.928706
Ran for 230550 frames Cell Events: 6653 mean_circ: 0.928687
Ran for 231550 frames Cell Events: 6686 mean_circ: 0.928734
Ran for 233050 frames Cell Events: 6727 mean_circ: 0.928792
Ran for 233550 frames Cell Events: 6739 mean_circ: 0.928798
Ran for 234050 frames Cell Events: 6751 mean_circ: 0.928795
Ran for 235550 frames Cell Events: 6810 mean_circ: 0.928765
Ran for 236550 frames Cell Events: 6842 mean_circ: 0.928767
Ran for 237550 frames Cell Events: 6876 mean_circ: 0.928766
Ran for 238550 frames Cell Events: 6912 mean_circ: 0.928757
Ran for 239550 frames Cell Events: 6937 mean_circ: 0.928752
Ran for 240050 frames Cell Events: 6948 mean_circ: 0.928724
Ran for 240550 frames Cell Events: 6956 mean_circ: 0.928719
Ran for 241550 frames Cell Events: 6979 mean_circ: 0.928657
Ran for 242050 frames Cell Events: 6997 mean_circ: 0.928649
Ran for 242550 frames Cell Events: 7013 mean_circ: 0.928656
Ran for 243550 frames Cell Events: 7048 mean_circ: 0.928625
End this measurment..
Stopping time: 2.18762e+009
****************************************
Measurement finished
Mean Circularitiy: 0.928621
Mean Area: 749.942
Mean FL-1 intensity: 3004.93
Mean FL-2 intensity: 3754.73
Mean FL-3 intensity: 0
frames processed: 242038
Total Time: 121991ms
Number of Runs (new bg img) 0
Frames collected: 243833
Skipped frames: 46337
Rescued Frames: 4510788
Samples DAQ: 60958281
Samples written: 121916466
Samples per Channel: 60958233
FPS: 1998.77
Samples per second: 499693
Expected samples/s: 500000
Events: 7057
Events per second: 57.8484
jumped back (PMT): 3835
#Frames read, frequency
# 0 frames :367775
# 1 frames :101799
# 2 frames :4129
# 3 frames :2283
# 4 frames :1304
# 5 frames :896
# 6 frames :665
# 7 frames :1117
# 8 frames :1107
# 9 frames :857
# 10 frames :730
# 11 frames :715
# 12 frames :612
# 13 frames :466
# 14 frames :347
# 15 frames :284
# 16 frames :223
# 17 frames :263
# 18 frames :221
# 19 frames :260
# 20 frames :220
# 21 frames :192
# 22 frames :139
# 23 frames :122
# 24 frames :79
# 25 frames :57
# 26 frames :60
# 27 frames :41
# 28 frames :45
# 29 frames :32
# 30 frames :37
# 31 frames :30
# 32 frames :24
# 33 frames :17
# 34 frames :24
# 35 frames :26
# 36 frames :16
# 37 frames :10
# 38 frames :11
# 39 frames :11
# 40 frames :18
# 41 frames :16
# 42 frames :3
# 43 frames :6
# 44 frames :5
# 45 frames :7
# 46 frames :7
# 47 frames :1
# 48 frames :2
# 49 frames :4
# 50 frames :2
# 51 frames :2
# 52 frames :6
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 56 frames :4
# 57 frames :3
# 60 frames :2
# 63 frames :2
# 69 frames :1
# 70 frames :2
# 71 frames :1
# 95 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.8172
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0