Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.42.02
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "090ffef25cb83585678739a267a33b2c",
"name": "M1_20170620_1603_dsRNA_W3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:42:24"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8202 (max 8203) in '8202'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.909
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.64756e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 37 mean_circ: 0.947173
Ran for 1550 frames Cell Events: 50 mean_circ: 0.944608
Ran for 4050 frames Cell Events: 123 mean_circ: 0.948135
Ran for 5050 frames Cell Events: 161 mean_circ: 0.948425
Ran for 5550 frames Cell Events: 171 mean_circ: 0.948926
Ran for 6050 frames Cell Events: 186 mean_circ: 0.949239
Ran for 6550 frames Cell Events: 196 mean_circ: 0.948101
Ran for 7050 frames Cell Events: 205 mean_circ: 0.948577
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Ran for 8550 frames Cell Events: 264 mean_circ: 0.95021
Ran for 9050 frames Cell Events: 277 mean_circ: 0.950442
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Ran for 74550 frames Cell Events: 2384 mean_circ: 0.946231
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Ran for 83550 frames Cell Events: 2712 mean_circ: 0.946426
Ran for 84050 frames Cell Events: 2728 mean_circ: 0.946425
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Ran for 85550 frames Cell Events: 2791 mean_circ: 0.94647
Ran for 86050 frames Cell Events: 2808 mean_circ: 0.946472
Ran for 87050 frames Cell Events: 2836 mean_circ: 0.9464
Ran for 87550 frames Cell Events: 2848 mean_circ: 0.946416
Ran for 88550 frames Cell Events: 2885 mean_circ: 0.946471
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Ran for 91550 frames Cell Events: 2992 mean_circ: 0.946305
Ran for 93050 frames Cell Events: 3037 mean_circ: 0.946366
Ran for 94050 frames Cell Events: 3076 mean_circ: 0.946372
Ran for 94550 frames Cell Events: 3092 mean_circ: 0.946348
Ran for 95050 frames Cell Events: 3108 mean_circ: 0.946375
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Ran for 96050 frames Cell Events: 3136 mean_circ: 0.946278
Ran for 97050 frames Cell Events: 3165 mean_circ: 0.946171
Ran for 97550 frames Cell Events: 3179 mean_circ: 0.946132
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Ran for 100550 frames Cell Events: 3270 mean_circ: 0.946212
Ran for 101050 frames Cell Events: 3282 mean_circ: 0.946245
Ran for 101550 frames Cell Events: 3307 mean_circ: 0.9463
Ran for 102050 frames Cell Events: 3323 mean_circ: 0.946309
Ran for 102550 frames Cell Events: 3341 mean_circ: 0.946352
Ran for 103050 frames Cell Events: 3358 mean_circ: 0.946336
Ran for 104050 frames Cell Events: 3388 mean_circ: 0.946314
Ran for 104550 frames Cell Events: 3402 mean_circ: 0.946355
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Ran for 106550 frames Cell Events: 3457 mean_circ: 0.94624
Ran for 107550 frames Cell Events: 3490 mean_circ: 0.946188
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Ran for 120050 frames Cell Events: 3866 mean_circ: 0.946141
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Ran for 121050 frames Cell Events: 3891 mean_circ: 0.946185
Ran for 121550 frames Cell Events: 3902 mean_circ: 0.946212
Ran for 123550 frames Cell Events: 3974 mean_circ: 0.946145
Ran for 124050 frames Cell Events: 3984 mean_circ: 0.946159
Ran for 124550 frames Cell Events: 4003 mean_circ: 0.946117
Ran for 125550 frames Cell Events: 4039 mean_circ: 0.94606
Ran for 126550 frames Cell Events: 4065 mean_circ: 0.946101
Ran for 127050 frames Cell Events: 4075 mean_circ: 0.94609
Ran for 127550 frames Cell Events: 4095 mean_circ: 0.946088
Ran for 128050 frames Cell Events: 4114 mean_circ: 0.946144
Ran for 128550 frames Cell Events: 4137 mean_circ: 0.946097
Ran for 130050 frames Cell Events: 4190 mean_circ: 0.946103
Ran for 130550 frames Cell Events: 4202 mean_circ: 0.946106
Ran for 131550 frames Cell Events: 4230 mean_circ: 0.946183
Ran for 132050 frames Cell Events: 4243 mean_circ: 0.946209
Ran for 132550 frames Cell Events: 4260 mean_circ: 0.946204
Ran for 134050 frames Cell Events: 4305 mean_circ: 0.94621
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Ran for 136050 frames Cell Events: 4363 mean_circ: 0.946241
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Ran for 137550 frames Cell Events: 4421 mean_circ: 0.946156
Ran for 138050 frames Cell Events: 4432 mean_circ: 0.946182
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Ran for 141050 frames Cell Events: 4531 mean_circ: 0.946132
Ran for 142050 frames Cell Events: 4571 mean_circ: 0.946101
Ran for 142550 frames Cell Events: 4582 mean_circ: 0.946093
Ran for 143550 frames Cell Events: 4622 mean_circ: 0.946055
Ran for 144050 frames Cell Events: 4640 mean_circ: 0.94606
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Ran for 146050 frames Cell Events: 4705 mean_circ: 0.946078
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Ran for 147550 frames Cell Events: 4746 mean_circ: 0.946058
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Ran for 152050 frames Cell Events: 4933 mean_circ: 0.946008
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Ran for 171550 frames Cell Events: 5533 mean_circ: 0.946056
Ran for 172050 frames Cell Events: 5547 mean_circ: 0.946085
Ran for 173550 frames Cell Events: 5595 mean_circ: 0.946053
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Ran for 174550 frames Cell Events: 5628 mean_circ: 0.946028
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Ran for 176550 frames Cell Events: 5696 mean_circ: 0.945948
Ran for 177050 frames Cell Events: 5714 mean_circ: 0.945903
Ran for 178550 frames Cell Events: 5751 mean_circ: 0.945951
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Ran for 192050 frames Cell Events: 6196 mean_circ: 0.9459
Ran for 192550 frames Cell Events: 6209 mean_circ: 0.945895
Ran for 193050 frames Cell Events: 6232 mean_circ: 0.945881
Ran for 193550 frames Cell Events: 6247 mean_circ: 0.945887
Ran for 194050 frames Cell Events: 6256 mean_circ: 0.945905
Ran for 194550 frames Cell Events: 6280 mean_circ: 0.945934
Ran for 195550 frames Cell Events: 6309 mean_circ: 0.945949
Ran for 196050 frames Cell Events: 6323 mean_circ: 0.94593
Ran for 197050 frames Cell Events: 6347 mean_circ: 0.945924
Ran for 197550 frames Cell Events: 6363 mean_circ: 0.945905
Ran for 198050 frames Cell Events: 6387 mean_circ: 0.945891
Ran for 199050 frames Cell Events: 6416 mean_circ: 0.945915
Ran for 200050 frames Cell Events: 6448 mean_circ: 0.945931
Ran for 200550 frames Cell Events: 6464 mean_circ: 0.945932
Ran for 201050 frames Cell Events: 6474 mean_circ: 0.945887
Ran for 201550 frames Cell Events: 6496 mean_circ: 0.945872
Ran for 202050 frames Cell Events: 6511 mean_circ: 0.945917
Ran for 203550 frames Cell Events: 6563 mean_circ: 0.945857
Ran for 204050 frames Cell Events: 6580 mean_circ: 0.945851
Ran for 204550 frames Cell Events: 6599 mean_circ: 0.945848
Ran for 206050 frames Cell Events: 6663 mean_circ: 0.945777
Ran for 207050 frames Cell Events: 6700 mean_circ: 0.945725
Ran for 207550 frames Cell Events: 6714 mean_circ: 0.945729
Ran for 210550 frames Cell Events: 6814 mean_circ: 0.945777
Ran for 211550 frames Cell Events: 6846 mean_circ: 0.945768
Ran for 212050 frames Cell Events: 6858 mean_circ: 0.945785
Ran for 213050 frames Cell Events: 6883 mean_circ: 0.945727
Ran for 214050 frames Cell Events: 6919 mean_circ: 0.945736
Ran for 214550 frames Cell Events: 6946 mean_circ: 0.945738
Ran for 215050 frames Cell Events: 6967 mean_circ: 0.945801
Ran for 216550 frames Cell Events: 7023 mean_circ: 0.945822
Ran for 217050 frames Cell Events: 7037 mean_circ: 0.945815
Ran for 218050 frames Cell Events: 7065 mean_circ: 0.945777
Ran for 218550 frames Cell Events: 7073 mean_circ: 0.945749
Ran for 220050 frames Cell Events: 7125 mean_circ: 0.945757
Ran for 220550 frames Cell Events: 7144 mean_circ: 0.945771
Ran for 221550 frames Cell Events: 7178 mean_circ: 0.945809
Ran for 222550 frames Cell Events: 7215 mean_circ: 0.94582
Ran for 223550 frames Cell Events: 7241 mean_circ: 0.945775
Ran for 224050 frames Cell Events: 7255 mean_circ: 0.945781
Ran for 224550 frames Cell Events: 7271 mean_circ: 0.945744
Ran for 226050 frames Cell Events: 7322 mean_circ: 0.945795
Ran for 226550 frames Cell Events: 7336 mean_circ: 0.945802
Ran for 227050 frames Cell Events: 7357 mean_circ: 0.945768
Ran for 227550 frames Cell Events: 7388 mean_circ: 0.945816
Ran for 228050 frames Cell Events: 7404 mean_circ: 0.94585
Ran for 228550 frames Cell Events: 7415 mean_circ: 0.945856
Ran for 229050 frames Cell Events: 7431 mean_circ: 0.945877
Ran for 230550 frames Cell Events: 7479 mean_circ: 0.945915
Ran for 231550 frames Cell Events: 7506 mean_circ: 0.945916
Ran for 232050 frames Cell Events: 7518 mean_circ: 0.945907
Ran for 232550 frames Cell Events: 7549 mean_circ: 0.945868
Ran for 233050 frames Cell Events: 7560 mean_circ: 0.945866
Ran for 234050 frames Cell Events: 7604 mean_circ: 0.945821
Ran for 235050 frames Cell Events: 7647 mean_circ: 0.94586
Ran for 235550 frames Cell Events: 7656 mean_circ: 0.945867
Ran for 236050 frames Cell Events: 7669 mean_circ: 0.945879
Ran for 237050 frames Cell Events: 7705 mean_circ: 0.945863
Ran for 237550 frames Cell Events: 7728 mean_circ: 0.945896
Ran for 238050 frames Cell Events: 7745 mean_circ: 0.945945
Ran for 239050 frames Cell Events: 7785 mean_circ: 0.945944
Ran for 240050 frames Cell Events: 7815 mean_circ: 0.945951
Ran for 241050 frames Cell Events: 7847 mean_circ: 0.945935
Ran for 241550 frames Cell Events: 7858 mean_circ: 0.945948
Ran for 242050 frames Cell Events: 7866 mean_circ: 0.945932
Ran for 242550 frames Cell Events: 7879 mean_circ: 0.945963
Ran for 245050 frames Cell Events: 7969 mean_circ: 0.94587
Ran for 246050 frames Cell Events: 7997 mean_circ: 0.945858
Ran for 246550 frames Cell Events: 8018 mean_circ: 0.945811
Ran for 247050 frames Cell Events: 8030 mean_circ: 0.945772
Ran for 247550 frames Cell Events: 8049 mean_circ: 0.945741
Ran for 248050 frames Cell Events: 8069 mean_circ: 0.945737
Ran for 248550 frames Cell Events: 8088 mean_circ: 0.945713
Ran for 249550 frames Cell Events: 8103 mean_circ: 0.945724
Ran for 250050 frames Cell Events: 8122 mean_circ: 0.945724
Ran for 250550 frames Cell Events: 8151 mean_circ: 0.94575
Ran for 251050 frames Cell Events: 8173 mean_circ: 0.945744
Ran for 251550 frames Cell Events: 8189 mean_circ: 0.945706
End this measurment..
Stopping time: 5.64883e+008
****************************************
Measurement finished
Mean Circularitiy: 0.945704
Mean Area: 642.285
Mean FL-1 intensity: 3964.63
Mean FL-2 intensity: 3535.51
Mean FL-3 intensity: 0
frames processed: 251318
Total Time: 126053ms
Number of Runs (new bg img) 0
Frames collected: 251893
Skipped frames: 42010
Rescued Frames: 2399555
Samples DAQ: 62973515
Samples written: 125946336
Samples per Channel: 62973168
FPS: 1998.3
Samples per second: 499575
Expected samples/s: 500000
Events: 8203
Events per second: 65.0755
jumped back (PMT): 6609
#Frames read, frequency
# 0 frames :345654
# 1 frames :138157
# 2 frames :7171
# 3 frames :1617
# 4 frames :1188
# 5 frames :429
# 6 frames :965
# 7 frames :3096
# 8 frames :1839
# 9 frames :1061
# 10 frames :622
# 11 frames :352
# 12 frames :375
# 13 frames :269
# 14 frames :168
# 15 frames :186
# 16 frames :140
# 17 frames :94
# 18 frames :75
# 19 frames :55
# 20 frames :33
# 21 frames :45
# 22 frames :45
# 23 frames :25
# 24 frames :19
# 25 frames :17
# 26 frames :11
# 27 frames :10
# 28 frames :7
# 29 frames :7
# 30 frames :3
# 31 frames :4
# 32 frames :1
# 33 frames :3
# 34 frames :1
# 37 frames :1
# 38 frames :2
# 39 frames :2
# 40 frames :1
# 41 frames :1
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 47 frames :1
# 48 frames :1
# 60 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.909
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0