Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.21.53
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "97fe6f69e7b0813c1ed52ac4576473eb",
"name": "M1_20170613_1720_dsRNA_X8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:22:14"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8595 (max 8596) in '8595'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6814
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53786e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 10 mean_circ: 0.941527
Ran for 1550 frames Cell Events: 40 mean_circ: 0.934873
Ran for 2050 frames Cell Events: 47 mean_circ: 0.939542
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Ran for 93550 frames Cell Events: 2922 mean_circ: 0.941619
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Ran for 96550 frames Cell Events: 3028 mean_circ: 0.941418
Ran for 97050 frames Cell Events: 3048 mean_circ: 0.94134
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Ran for 100550 frames Cell Events: 3160 mean_circ: 0.941322
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Ran for 106550 frames Cell Events: 3368 mean_circ: 0.941195
Ran for 107050 frames Cell Events: 3391 mean_circ: 0.941212
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Ran for 109050 frames Cell Events: 3452 mean_circ: 0.941094
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Ran for 121550 frames Cell Events: 3887 mean_circ: 0.940742
Ran for 122550 frames Cell Events: 3921 mean_circ: 0.940781
Ran for 123550 frames Cell Events: 3950 mean_circ: 0.940703
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Ran for 143050 frames Cell Events: 4646 mean_circ: 0.940696
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Ran for 146550 frames Cell Events: 4764 mean_circ: 0.940706
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Ran for 220050 frames Cell Events: 7538 mean_circ: 0.940245
Ran for 220550 frames Cell Events: 7550 mean_circ: 0.940206
Ran for 221050 frames Cell Events: 7561 mean_circ: 0.940193
Ran for 221550 frames Cell Events: 7580 mean_circ: 0.940163
Ran for 222050 frames Cell Events: 7593 mean_circ: 0.940186
Ran for 223550 frames Cell Events: 7652 mean_circ: 0.940171
Ran for 224050 frames Cell Events: 7669 mean_circ: 0.94012
Ran for 224550 frames Cell Events: 7680 mean_circ: 0.940104
Ran for 225550 frames Cell Events: 7724 mean_circ: 0.940038
Ran for 226050 frames Cell Events: 7745 mean_circ: 0.940042
Ran for 227050 frames Cell Events: 7792 mean_circ: 0.940055
Ran for 227550 frames Cell Events: 7815 mean_circ: 0.940052
Ran for 228050 frames Cell Events: 7826 mean_circ: 0.940054
Ran for 229050 frames Cell Events: 7860 mean_circ: 0.940063
Ran for 229550 frames Cell Events: 7884 mean_circ: 0.940089
Ran for 231050 frames Cell Events: 7946 mean_circ: 0.940072
Ran for 232050 frames Cell Events: 7982 mean_circ: 0.940083
Ran for 232550 frames Cell Events: 7996 mean_circ: 0.940089
Ran for 234550 frames Cell Events: 8089 mean_circ: 0.940014
Ran for 236050 frames Cell Events: 8148 mean_circ: 0.939993
Ran for 237050 frames Cell Events: 8189 mean_circ: 0.939899
Ran for 238550 frames Cell Events: 8254 mean_circ: 0.939909
Ran for 239050 frames Cell Events: 8273 mean_circ: 0.939948
Ran for 239550 frames Cell Events: 8288 mean_circ: 0.939958
Ran for 240050 frames Cell Events: 8317 mean_circ: 0.939935
Ran for 240550 frames Cell Events: 8343 mean_circ: 0.939888
Ran for 241050 frames Cell Events: 8358 mean_circ: 0.939879
Ran for 241550 frames Cell Events: 8386 mean_circ: 0.939904
Ran for 242050 frames Cell Events: 8400 mean_circ: 0.939879
Ran for 242550 frames Cell Events: 8439 mean_circ: 0.939906
Ran for 243550 frames Cell Events: 8473 mean_circ: 0.939931
Ran for 245050 frames Cell Events: 8543 mean_circ: 0.939898
Ran for 245550 frames Cell Events: 8561 mean_circ: 0.939892
Ran for 246050 frames Cell Events: 8577 mean_circ: 0.939898
Ran for 246550 frames Cell Events: 8595 mean_circ: 0.939893
End this measurment..
Stopping time: 2.53799e+009
****************************************
Measurement finished
Mean Circularitiy: 0.939895
Mean Area: 594.304
Mean FL-1 intensity: 3348.7
Mean FL-2 intensity: 3289.37
Mean FL-3 intensity: 0
frames processed: 245994
Total Time: 123347ms
Number of Runs (new bg img) 0
Frames collected: 246558
Skipped frames: 35889
Rescued Frames: 3944301
Samples DAQ: 61640692
Samples written: 123279978
Samples per Channel: 61639989
FPS: 1998.9
Samples per second: 499729
Expected samples/s: 500000
Events: 8596
Events per second: 69.6897
jumped back (PMT): 5574
#Frames read, frequency
# 0 frames :361244
# 1 frames :111926
# 2 frames :5809
# 3 frames :2016
# 4 frames :1284
# 5 frames :739
# 6 frames :1080
# 7 frames :2013
# 8 frames :1673
# 9 frames :1131
# 10 frames :747
# 11 frames :520
# 12 frames :471
# 13 frames :378
# 14 frames :320
# 15 frames :284
# 16 frames :245
# 17 frames :210
# 18 frames :162
# 19 frames :122
# 20 frames :92
# 21 frames :77
# 22 frames :83
# 23 frames :65
# 24 frames :54
# 25 frames :53
# 26 frames :34
# 27 frames :39
# 28 frames :27
# 29 frames :19
# 30 frames :24
# 31 frames :17
# 32 frames :17
# 33 frames :19
# 34 frames :9
# 35 frames :11
# 36 frames :12
# 37 frames :7
# 38 frames :10
# 39 frames :3
# 40 frames :5
# 41 frames :3
# 42 frames :6
# 45 frames :2
# 46 frames :4
# 47 frames :6
# 48 frames :2
# 50 frames :1
# 51 frames :2
# 54 frames :1
# 57 frames :2
# 62 frames :1
# 67 frames :1
# 78 frames :1
# 87 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6814
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0