Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.39.49
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3f998e4d36ff5361cd2527b0f9ae3c45",
"name": "M1_20170615_1416_dsRNA_Y2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:40:10"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8311 (max 8313) in '8311'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6868
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.2633e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 47 mean_circ: 0.934904
Ran for 1550 frames Cell Events: 85 mean_circ: 0.946025
Ran for 2050 frames Cell Events: 115 mean_circ: 0.949991
Ran for 3050 frames Cell Events: 149 mean_circ: 0.95038
Ran for 3550 frames Cell Events: 182 mean_circ: 0.951545
Ran for 5050 frames Cell Events: 269 mean_circ: 0.952394
Ran for 7050 frames Cell Events: 382 mean_circ: 0.952476
Ran for 7550 frames Cell Events: 411 mean_circ: 0.951942
Ran for 8550 frames Cell Events: 468 mean_circ: 0.952141
Ran for 9550 frames Cell Events: 526 mean_circ: 0.95295
Ran for 10050 frames Cell Events: 559 mean_circ: 0.952381
Ran for 10550 frames Cell Events: 584 mean_circ: 0.952899
Ran for 11050 frames Cell Events: 612 mean_circ: 0.953181
Ran for 12550 frames Cell Events: 703 mean_circ: 0.953706
Ran for 14050 frames Cell Events: 788 mean_circ: 0.95404
Ran for 16550 frames Cell Events: 900 mean_circ: 0.954361
Ran for 17550 frames Cell Events: 959 mean_circ: 0.954308
Ran for 18050 frames Cell Events: 983 mean_circ: 0.953933
Ran for 19550 frames Cell Events: 1086 mean_circ: 0.954152
Ran for 20550 frames Cell Events: 1144 mean_circ: 0.954073
Ran for 21050 frames Cell Events: 1164 mean_circ: 0.953894
Ran for 21550 frames Cell Events: 1192 mean_circ: 0.954015
Ran for 23050 frames Cell Events: 1271 mean_circ: 0.953752
Ran for 24550 frames Cell Events: 1345 mean_circ: 0.953832
Ran for 25050 frames Cell Events: 1372 mean_circ: 0.953948
Ran for 26550 frames Cell Events: 1477 mean_circ: 0.953981
Ran for 28050 frames Cell Events: 1556 mean_circ: 0.9543
Ran for 30050 frames Cell Events: 1685 mean_circ: 0.954439
Ran for 30550 frames Cell Events: 1718 mean_circ: 0.954451
Ran for 32550 frames Cell Events: 1849 mean_circ: 0.95482
Ran for 33050 frames Cell Events: 1879 mean_circ: 0.954928
Ran for 33550 frames Cell Events: 1907 mean_circ: 0.95486
Ran for 35050 frames Cell Events: 1988 mean_circ: 0.954802
Ran for 35550 frames Cell Events: 2022 mean_circ: 0.954857
Ran for 36550 frames Cell Events: 2079 mean_circ: 0.95469
Ran for 37550 frames Cell Events: 2142 mean_circ: 0.954616
Ran for 38050 frames Cell Events: 2170 mean_circ: 0.954598
Ran for 38550 frames Cell Events: 2191 mean_circ: 0.954519
Ran for 39550 frames Cell Events: 2268 mean_circ: 0.954518
Ran for 40550 frames Cell Events: 2339 mean_circ: 0.954407
Ran for 42050 frames Cell Events: 2412 mean_circ: 0.954259
Ran for 46050 frames Cell Events: 2643 mean_circ: 0.954179
Ran for 46550 frames Cell Events: 2684 mean_circ: 0.954179
Ran for 47050 frames Cell Events: 2707 mean_circ: 0.953966
Ran for 47550 frames Cell Events: 2733 mean_circ: 0.953865
Ran for 48050 frames Cell Events: 2763 mean_circ: 0.953899
Ran for 50050 frames Cell Events: 2888 mean_circ: 0.953821
Ran for 50550 frames Cell Events: 2918 mean_circ: 0.953859
Ran for 51050 frames Cell Events: 2945 mean_circ: 0.953846
Ran for 52550 frames Cell Events: 3063 mean_circ: 0.953982
Ran for 53550 frames Cell Events: 3106 mean_circ: 0.954029
Ran for 54050 frames Cell Events: 3136 mean_circ: 0.953986
Ran for 55550 frames Cell Events: 3236 mean_circ: 0.953924
Ran for 56050 frames Cell Events: 3257 mean_circ: 0.953973
Ran for 57050 frames Cell Events: 3302 mean_circ: 0.953933
Ran for 58050 frames Cell Events: 3370 mean_circ: 0.953994
Ran for 58550 frames Cell Events: 3391 mean_circ: 0.953953
Ran for 61050 frames Cell Events: 3530 mean_circ: 0.953866
Ran for 62050 frames Cell Events: 3577 mean_circ: 0.953895
Ran for 63050 frames Cell Events: 3631 mean_circ: 0.9539
Ran for 64050 frames Cell Events: 3700 mean_circ: 0.953902
Ran for 66050 frames Cell Events: 3832 mean_circ: 0.953688
Ran for 67050 frames Cell Events: 3890 mean_circ: 0.953632
Ran for 68050 frames Cell Events: 3960 mean_circ: 0.953523
Ran for 68550 frames Cell Events: 3987 mean_circ: 0.953466
Ran for 69050 frames Cell Events: 4000 mean_circ: 0.953474
Ran for 69550 frames Cell Events: 4036 mean_circ: 0.953464
Ran for 70050 frames Cell Events: 4073 mean_circ: 0.953395
Ran for 70550 frames Cell Events: 4105 mean_circ: 0.953395
Ran for 71050 frames Cell Events: 4120 mean_circ: 0.953245
Ran for 71550 frames Cell Events: 4149 mean_circ: 0.95322
Ran for 73550 frames Cell Events: 4266 mean_circ: 0.953243
Ran for 78050 frames Cell Events: 4535 mean_circ: 0.953267
Ran for 79050 frames Cell Events: 4612 mean_circ: 0.953233
Ran for 79550 frames Cell Events: 4642 mean_circ: 0.953198
Ran for 80550 frames Cell Events: 4707 mean_circ: 0.953262
Ran for 81050 frames Cell Events: 4723 mean_circ: 0.953298
Ran for 81550 frames Cell Events: 4756 mean_circ: 0.953264
Ran for 82050 frames Cell Events: 4797 mean_circ: 0.953282
Ran for 87050 frames Cell Events: 5148 mean_circ: 0.953101
Ran for 89550 frames Cell Events: 5292 mean_circ: 0.953133
Ran for 90050 frames Cell Events: 5332 mean_circ: 0.953133
Ran for 92050 frames Cell Events: 5463 mean_circ: 0.953022
Ran for 92550 frames Cell Events: 5505 mean_circ: 0.953014
Ran for 93550 frames Cell Events: 5569 mean_circ: 0.952854
Ran for 94050 frames Cell Events: 5597 mean_circ: 0.952854
Ran for 97050 frames Cell Events: 5770 mean_circ: 0.952814
Ran for 98050 frames Cell Events: 5827 mean_circ: 0.952861
Ran for 98550 frames Cell Events: 5851 mean_circ: 0.952838
Ran for 99050 frames Cell Events: 5881 mean_circ: 0.952831
Ran for 100550 frames Cell Events: 5975 mean_circ: 0.952675
Ran for 101050 frames Cell Events: 5997 mean_circ: 0.952688
Ran for 101550 frames Cell Events: 6032 mean_circ: 0.952673
Ran for 102050 frames Cell Events: 6070 mean_circ: 0.95266
Ran for 102550 frames Cell Events: 6097 mean_circ: 0.952636
Ran for 103050 frames Cell Events: 6128 mean_circ: 0.952637
Ran for 103550 frames Cell Events: 6161 mean_circ: 0.952633
Ran for 105550 frames Cell Events: 6299 mean_circ: 0.952751
Ran for 106050 frames Cell Events: 6334 mean_circ: 0.952751
Ran for 107050 frames Cell Events: 6390 mean_circ: 0.95271
Ran for 108050 frames Cell Events: 6443 mean_circ: 0.952733
Ran for 111550 frames Cell Events: 6683 mean_circ: 0.952658
Ran for 112050 frames Cell Events: 6718 mean_circ: 0.95267
Ran for 112550 frames Cell Events: 6752 mean_circ: 0.952654
Ran for 113050 frames Cell Events: 6780 mean_circ: 0.952683
Ran for 114050 frames Cell Events: 6837 mean_circ: 0.952656
Ran for 116050 frames Cell Events: 6956 mean_circ: 0.952696
Ran for 116550 frames Cell Events: 6987 mean_circ: 0.952689
Ran for 117550 frames Cell Events: 7045 mean_circ: 0.952649
Ran for 118550 frames Cell Events: 7109 mean_circ: 0.952634
Ran for 119050 frames Cell Events: 7140 mean_circ: 0.952642
Ran for 120550 frames Cell Events: 7230 mean_circ: 0.95259
Ran for 122050 frames Cell Events: 7333 mean_circ: 0.95263
Ran for 124050 frames Cell Events: 7451 mean_circ: 0.952648
Ran for 124550 frames Cell Events: 7475 mean_circ: 0.952616
Ran for 126050 frames Cell Events: 7559 mean_circ: 0.952484
Ran for 128550 frames Cell Events: 7722 mean_circ: 0.952483
Ran for 129050 frames Cell Events: 7754 mean_circ: 0.952458
Ran for 131050 frames Cell Events: 7846 mean_circ: 0.952431
Ran for 132550 frames Cell Events: 7935 mean_circ: 0.952397
Ran for 133050 frames Cell Events: 7964 mean_circ: 0.952387
Ran for 134550 frames Cell Events: 8057 mean_circ: 0.952371
Ran for 135550 frames Cell Events: 8120 mean_circ: 0.952374
Ran for 136550 frames Cell Events: 8164 mean_circ: 0.952328
Ran for 137550 frames Cell Events: 8240 mean_circ: 0.952284
Ran for 138050 frames Cell Events: 8260 mean_circ: 0.952281
End this measurment..
Stopping time: 1.26399e+008
****************************************
Measurement finished
Mean Circularitiy: 0.952262
Mean Area: 617.158
Mean FL-1 intensity: 3661.51
Mean FL-2 intensity: 2766.42
Mean FL-3 intensity: 0
frames processed: 137225
Total Time: 69505.4ms
Number of Runs (new bg img) 0
Frames collected: 138843
Skipped frames: 1618
Rescued Frames: 83808
Samples DAQ: 34712844
Samples written: 69421258
Samples per Channel: 34710629
FPS: 1997.58
Samples per second: 499394
Expected samples/s: 500000
Events: 8313
Events per second: 119.602
jumped back (PMT): 3485
#Frames read, frequency
# 0 frames :224094
# 1 frames :41904
# 2 frames :2529
# 3 frames :972
# 4 frames :647
# 5 frames :332
# 6 frames :502
# 7 frames :1775
# 8 frames :602
# 9 frames :600
# 10 frames :793
# 11 frames :369
# 12 frames :309
# 13 frames :224
# 14 frames :166
# 15 frames :239
# 16 frames :217
# 17 frames :190
# 18 frames :137
# 19 frames :122
# 20 frames :92
# 21 frames :63
# 22 frames :85
# 23 frames :54
# 24 frames :56
# 25 frames :46
# 26 frames :45
# 27 frames :56
# 28 frames :36
# 29 frames :29
# 30 frames :23
# 31 frames :13
# 32 frames :18
# 33 frames :25
# 34 frames :25
# 35 frames :23
# 36 frames :17
# 37 frames :12
# 38 frames :11
# 39 frames :9
# 40 frames :7
# 41 frames :10
# 42 frames :6
# 43 frames :6
# 44 frames :5
# 45 frames :5
# 46 frames :3
# 47 frames :3
# 48 frames :1
# 49 frames :4
# 51 frames :1
# 52 frames :2
# 53 frames :4
# 54 frames :2
# 55 frames :3
# 57 frames :1
# 58 frames :2
# 59 frames :1
# 60 frames :1
# 61 frames :1
# 63 frames :3
# 64 frames :1
# 65 frames :1
# 66 frames :1
# 74 frames :1
# 83 frames :1
# 84 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6868
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0