Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.20.58
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c5399f4b7dc18ae059e6f4bb31e5c637",
"name": "M1_20170613_1702_dsRNA_Y4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:21:19"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8422 (max 8423) in '8422'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6329
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53674e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.948841
Ran for 1050 frames Cell Events: 35 mean_circ: 0.943066
Ran for 1550 frames Cell Events: 56 mean_circ: 0.933908
Ran for 2050 frames Cell Events: 69 mean_circ: 0.933856
Ran for 3050 frames Cell Events: 104 mean_circ: 0.934751
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Ran for 122550 frames Cell Events: 4130 mean_circ: 0.931123
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Ran for 126050 frames Cell Events: 4263 mean_circ: 0.931021
Ran for 127050 frames Cell Events: 4290 mean_circ: 0.931055
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Ran for 199550 frames Cell Events: 7162 mean_circ: 0.931249
Ran for 200050 frames Cell Events: 7183 mean_circ: 0.931241
Ran for 200550 frames Cell Events: 7202 mean_circ: 0.931169
Ran for 202050 frames Cell Events: 7267 mean_circ: 0.931209
Ran for 202550 frames Cell Events: 7282 mean_circ: 0.931217
Ran for 203550 frames Cell Events: 7318 mean_circ: 0.931235
Ran for 204050 frames Cell Events: 7339 mean_circ: 0.931248
Ran for 205050 frames Cell Events: 7385 mean_circ: 0.931259
Ran for 205550 frames Cell Events: 7402 mean_circ: 0.931238
Ran for 206050 frames Cell Events: 7418 mean_circ: 0.931243
Ran for 207050 frames Cell Events: 7463 mean_circ: 0.931292
Ran for 207550 frames Cell Events: 7485 mean_circ: 0.931345
Ran for 208050 frames Cell Events: 7506 mean_circ: 0.931352
Ran for 208550 frames Cell Events: 7528 mean_circ: 0.931378
Ran for 209550 frames Cell Events: 7571 mean_circ: 0.931348
Ran for 210050 frames Cell Events: 7598 mean_circ: 0.931339
Ran for 210550 frames Cell Events: 7618 mean_circ: 0.931375
Ran for 212050 frames Cell Events: 7687 mean_circ: 0.931433
Ran for 212550 frames Cell Events: 7704 mean_circ: 0.931461
Ran for 213550 frames Cell Events: 7761 mean_circ: 0.931425
Ran for 214550 frames Cell Events: 7799 mean_circ: 0.93143
Ran for 215050 frames Cell Events: 7822 mean_circ: 0.931481
Ran for 215550 frames Cell Events: 7842 mean_circ: 0.931539
Ran for 217550 frames Cell Events: 7911 mean_circ: 0.931509
Ran for 218050 frames Cell Events: 7925 mean_circ: 0.931522
Ran for 218550 frames Cell Events: 7947 mean_circ: 0.931512
Ran for 219050 frames Cell Events: 7957 mean_circ: 0.931486
Ran for 220050 frames Cell Events: 8001 mean_circ: 0.931481
Ran for 221050 frames Cell Events: 8034 mean_circ: 0.931402
Ran for 221550 frames Cell Events: 8054 mean_circ: 0.931411
Ran for 222050 frames Cell Events: 8080 mean_circ: 0.931415
Ran for 223050 frames Cell Events: 8117 mean_circ: 0.931453
Ran for 223550 frames Cell Events: 8153 mean_circ: 0.931417
Ran for 224050 frames Cell Events: 8175 mean_circ: 0.931407
Ran for 224550 frames Cell Events: 8198 mean_circ: 0.931448
Ran for 225050 frames Cell Events: 8217 mean_circ: 0.931492
Ran for 225550 frames Cell Events: 8240 mean_circ: 0.93153
Ran for 226050 frames Cell Events: 8257 mean_circ: 0.931503
Ran for 226550 frames Cell Events: 8272 mean_circ: 0.931508
Ran for 227050 frames Cell Events: 8286 mean_circ: 0.931504
Ran for 227550 frames Cell Events: 8304 mean_circ: 0.93147
Ran for 228050 frames Cell Events: 8321 mean_circ: 0.931484
Ran for 228550 frames Cell Events: 8343 mean_circ: 0.931471
Ran for 229050 frames Cell Events: 8357 mean_circ: 0.931471
Ran for 230550 frames Cell Events: 8419 mean_circ: 0.931457
End this measurment..
Stopping time: 2.53686e+009
****************************************
Measurement finished
Mean Circularitiy: 0.931439
Mean Area: 747.732
Mean FL-1 intensity: 3278.38
Mean FL-2 intensity: 3454.33
Mean FL-3 intensity: 0
frames processed: 228921
Total Time: 115379ms
Number of Runs (new bg img) 0
Frames collected: 230595
Skipped frames: 33708
Rescued Frames: 3738167
Samples DAQ: 57651258
Samples written: 115297664
Samples per Channel: 57648832
FPS: 1998.59
Samples per second: 499649
Expected samples/s: 500000
Events: 8423
Events per second: 73.0031
jumped back (PMT): 4777
#Frames read, frequency
# 0 frames :331119
# 1 frames :112623
# 2 frames :5047
# 3 frames :1639
# 4 frames :1114
# 5 frames :466
# 6 frames :1099
# 7 frames :1864
# 8 frames :1604
# 9 frames :985
# 10 frames :701
# 11 frames :487
# 12 frames :428
# 13 frames :283
# 14 frames :253
# 15 frames :230
# 16 frames :214
# 17 frames :160
# 18 frames :136
# 19 frames :89
# 20 frames :75
# 21 frames :79
# 22 frames :64
# 23 frames :37
# 24 frames :35
# 25 frames :30
# 26 frames :30
# 27 frames :22
# 28 frames :22
# 29 frames :22
# 30 frames :19
# 31 frames :12
# 32 frames :13
# 33 frames :17
# 34 frames :9
# 35 frames :10
# 36 frames :8
# 37 frames :6
# 38 frames :6
# 39 frames :6
# 40 frames :3
# 41 frames :5
# 43 frames :6
# 44 frames :4
# 45 frames :3
# 46 frames :1
# 47 frames :3
# 48 frames :2
# 49 frames :5
# 50 frames :2
# 51 frames :1
# 53 frames :2
# 55 frames :2
# 59 frames :1
# 62 frames :2
# 63 frames :1
# 97 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6329
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0