Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.40.23
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "03befaf63a2d384e8f2402975bc870e4",
"name": "M1_20170519_1632_dsRNA_L8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:40:58"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.4749
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.74991e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 40 mean_circ: 0.893359
Ran for 2050 frames Cell Events: 90 mean_circ: 0.898821
Ran for 2550 frames Cell Events: 102 mean_circ: 0.900351
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Ran for 4550 frames Cell Events: 167 mean_circ: 0.898441
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Ran for 7050 frames Cell Events: 291 mean_circ: 0.900039
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Ran for 74050 frames Cell Events: 3193 mean_circ: 0.896614
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Ran for 83050 frames Cell Events: 3669 mean_circ: 0.896938
Ran for 84550 frames Cell Events: 3740 mean_circ: 0.896814
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Ran for 92050 frames Cell Events: 4044 mean_circ: 0.897148
Ran for 93050 frames Cell Events: 4108 mean_circ: 0.897133
Ran for 96050 frames Cell Events: 4282 mean_circ: 0.896944
Ran for 96550 frames Cell Events: 4306 mean_circ: 0.896851
Ran for 97050 frames Cell Events: 4330 mean_circ: 0.896869
Ran for 97550 frames Cell Events: 4346 mean_circ: 0.896862
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Ran for 100050 frames Cell Events: 4467 mean_circ: 0.896892
Ran for 100550 frames Cell Events: 4493 mean_circ: 0.896892
Ran for 101050 frames Cell Events: 4510 mean_circ: 0.896917
Ran for 102050 frames Cell Events: 4553 mean_circ: 0.89696
Ran for 102550 frames Cell Events: 4572 mean_circ: 0.896899
Ran for 103050 frames Cell Events: 4595 mean_circ: 0.89682
Ran for 104050 frames Cell Events: 4632 mean_circ: 0.896884
Ran for 106050 frames Cell Events: 4721 mean_circ: 0.896652
Ran for 107050 frames Cell Events: 4771 mean_circ: 0.896578
Ran for 107550 frames Cell Events: 4807 mean_circ: 0.89662
Ran for 108050 frames Cell Events: 4835 mean_circ: 0.8965
Ran for 108550 frames Cell Events: 4871 mean_circ: 0.896505
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Ran for 111550 frames Cell Events: 4997 mean_circ: 0.896478
Ran for 112050 frames Cell Events: 5022 mean_circ: 0.896419
Ran for 113050 frames Cell Events: 5069 mean_circ: 0.896378
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Ran for 122550 frames Cell Events: 5487 mean_circ: 0.896078
Ran for 123550 frames Cell Events: 5530 mean_circ: 0.896122
Ran for 124050 frames Cell Events: 5550 mean_circ: 0.896083
Ran for 125050 frames Cell Events: 5564 mean_circ: 0.896063
Ran for 125550 frames Cell Events: 5579 mean_circ: 0.896026
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Ran for 126550 frames Cell Events: 5612 mean_circ: 0.896027
Ran for 128050 frames Cell Events: 5668 mean_circ: 0.895998
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Ran for 138050 frames Cell Events: 6111 mean_circ: 0.895533
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Ran for 141550 frames Cell Events: 6283 mean_circ: 0.895674
Ran for 144050 frames Cell Events: 6436 mean_circ: 0.895527
Ran for 144550 frames Cell Events: 6471 mean_circ: 0.89558
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Ran for 146050 frames Cell Events: 6540 mean_circ: 0.895509
Ran for 146550 frames Cell Events: 6560 mean_circ: 0.895532
Ran for 147050 frames Cell Events: 6581 mean_circ: 0.895532
Ran for 147550 frames Cell Events: 6604 mean_circ: 0.895502
Ran for 149050 frames Cell Events: 6658 mean_circ: 0.895436
Ran for 149550 frames Cell Events: 6684 mean_circ: 0.895453
Ran for 150050 frames Cell Events: 6706 mean_circ: 0.895443
Ran for 150550 frames Cell Events: 6727 mean_circ: 0.895492
Ran for 151550 frames Cell Events: 6778 mean_circ: 0.895504
Ran for 152550 frames Cell Events: 6827 mean_circ: 0.895455
Ran for 153050 frames Cell Events: 6852 mean_circ: 0.89548
Ran for 153550 frames Cell Events: 6871 mean_circ: 0.895474
Ran for 154550 frames Cell Events: 6925 mean_circ: 0.89545
Ran for 156050 frames Cell Events: 7001 mean_circ: 0.895348
Ran for 156550 frames Cell Events: 7012 mean_circ: 0.895374
Ran for 157050 frames Cell Events: 7036 mean_circ: 0.895372
Ran for 157550 frames Cell Events: 7064 mean_circ: 0.895394
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Ran for 216550 frames Cell Events: 9761 mean_circ: 0.894624
Ran for 217550 frames Cell Events: 9811 mean_circ: 0.894646
Ran for 218050 frames Cell Events: 9840 mean_circ: 0.894644
Ran for 219550 frames Cell Events: 9928 mean_circ: 0.894552
Ran for 220050 frames Cell Events: 9942 mean_circ: 0.894591
Ran for 220550 frames Cell Events: 9959 mean_circ: 0.894605
Ran for 221050 frames Cell Events: 9980 mean_circ: 0.894622
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 3.75102e+008
****************************************
Measurement finished
Mean Circularitiy: 0.894585
Mean Area: 620.184
Mean FL-1 intensity: 2323.36
Mean FL-2 intensity: 3523.88
Mean FL-3 intensity: 0
frames processed: 217631
Total Time: 110760ms
Number of Runs (new bg img) 0
Frames collected: 221395
Skipped frames: 64964
Rescued Frames: 3118870
Samples DAQ: 55351751
Samples written: 110697516
Samples per Channel: 55348758
FPS: 1998.87
Samples per second: 499717
Expected samples/s: 500000
Events: 10001
Events per second: 90.2942
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :320113
# 1 frames :105262
# 2 frames :4808
# 3 frames :1402
# 4 frames :776
# 5 frames :282
# 6 frames :2380
# 7 frames :2482
# 8 frames :1422
# 9 frames :940
# 10 frames :537
# 11 frames :320
# 12 frames :299
# 13 frames :291
# 14 frames :251
# 15 frames :228
# 16 frames :181
# 17 frames :136
# 18 frames :91
# 19 frames :82
# 20 frames :70
# 21 frames :56
# 22 frames :45
# 23 frames :38
# 24 frames :39
# 25 frames :27
# 26 frames :14
# 27 frames :27
# 28 frames :17
# 29 frames :10
# 30 frames :9
# 31 frames :10
# 32 frames :17
# 33 frames :7
# 34 frames :3
# 35 frames :4
# 36 frames :4
# 37 frames :7
# 38 frames :5
# 39 frames :5
# 40 frames :7
# 41 frames :2
# 45 frames :1
# 46 frames :1
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 53 frames :1
# 54 frames :1
# 57 frames :1
# 58 frames :2
# 61 frames :1
# 70 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.4749
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0