Polyacrylamide hydrogel beads from the same production batch were measured
with real-time deformability cytometry (RT-DC) at different flow rates (0.04,
0.08, and 0.12 µL/s). The beads were designed to have a Young's modulus of
approximately 4.3 ± 1.5 kPa. These measurements were used to illustrate the
correction of systematic errors for the computation of the Young's modulus in
RT-DC (figure 10 in [1]). The reservoir measurement was added for the sake of
completeness. The files were converted from the .tdms file format to the .rtdc
file format using DCKit 0.10.0 [2] and dclab 0.27.8 [3].
Before analysis, the data should be filtered for porosity (1.0 < area_ratio <
1.1) and deformation (0 < deform < 0.035). This is necessary, because events
were not always detected correctly (incorrect contour data) due to lack of
imaging contrast.
References:
[1] Christoph Herold. Mapping of Deformation to Apparent Young’s Modulus in
Real-Time Deformability Cytometry. ArXiv e-prints 1704.00572 [cond-mat.soft],
2017. arXiv:1704.00572v1.
[2] https://github.com/ZELLMECHANIK-DRESDEN/DCKit
[3] https://github.com/ZellMechanik-Dresden/dclab
SHA256 sums:
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2016-03-02_M3_0.0400uls_22f26fe5.rtdc
db5859d009396c697f1087479913443309e39414ab9d4ccafa3097d229e2e31d
2016-03-02_M4_0.0800uls_1e1e8234.rtdc
16c7ca085eeae8590ddc3eb75069ac91cb3d5f5e8d3ec32d641aa4f26d09cdd0
2016-03-02_M5_0.1200uls_d387ab27.rtdc
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2016-03-02_M6_reservoir_efe92abf.rtdc