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Polyacrylamide beads measured with RT-DC at different flow rates

Polyacrylamide hydrogel beads from the same production batch were measured with real-time deformability cytometry (RT-DC) at different flow rates (0.04, 0.08, and 0.12 µL/s). The beads were designed to have a Young's modulus of approximately 4.3 ± 1.5 kPa. These measurements were used to illustrate the correction of systematic errors for the computation of the Young's modulus in RT-DC (figure 10 in [1]). The reservoir measurement was added for the sake of completeness. The files were converted from the .tdms file format to the .rtdc file format using DCKit 0.10.0 [2] and dclab 0.27.8 [3].

Before analysis, the data should be filtered for porosity (1.0 < area_ratio < 1.1) and deformation (0 < deform < 0.035). This is necessary, because events were not always detected correctly (incorrect contour data) due to lack of imaging contrast.

References:
[1] Christoph Herold. Mapping of Deformation to Apparent Young’s Modulus in Real-Time Deformability Cytometry. ArXiv e-prints 1704.00572 [cond-mat.soft], 2017. arXiv:1704.00572v1.
[2] https://github.com/ZELLMECHANIK-DRESDEN/DCKit
[3] https://github.com/ZellMechanik-Dresden/dclab

SHA256 sums:
7c7e262113384020cab970652d62ac1cccc61d10c89969c1f8fa2c55ec933b35 2016-03-02_M3_0.0400uls_22f26fe5.rtdc
db5859d009396c697f1087479913443309e39414ab9d4ccafa3097d229e2e31d 2016-03-02_M4_0.0800uls_1e1e8234.rtdc
16c7ca085eeae8590ddc3eb75069ac91cb3d5f5e8d3ec32d641aa4f26d09cdd0 2016-03-02_M5_0.1200uls_d387ab27.rtdc
379e8d67fe88861fb8170c3f535f4c7ec34cc52a71e12f7c0d41bbf0545e0a02 2016-03-02_M6_reservoir_efe92abf.rtdc

Data and Resources

Additional Info

Field Value
Last Updated September 16, 2020, 19:01 (+0200)
Created September 16, 2020, 18:02 (+0200)
Authors Paul Müller, Christoph Herold, Maik Herbig, Jochen Guck
DOI 10.6084/m9.figshare.12721436.v1
References arxiv:1704.00572 https://github.com/ZELLMECHANIK-DRESDEN/DCKit https://github.com/ZellMechanik-Dresden/dclab
License Creative Commons Public Domain Dedication
Dataset ID c7a98a04-4e0a-98a7-fb0b-eca379d1f219